摘要
目的探讨免疫细胞因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)在肝癌大鼠模型肝硬化期的变化及作用。方法选取雄性Wistar大鼠70只,随机分为两组,正常组(35只):饮用灭菌食用水;实验组(35只):饮用灭菌食用水配制浓度0.1mg/mL的二乙基亚硝胺(DEN)溶液,分别于第5、7、9、11w检测两组大鼠外周血IL-1β、IL-6和TNF-α等免疫指标的变化。结果与正常组相比,实验组大鼠血清IL-1β、IL-6和TNF-α水平均升高,差异均具有统计学意义(P<0.01)。结论肝癌大鼠模型肝硬化期免疫细胞因子IL-1β、IL-6和TNF-α的异常变化可促进肝硬化进一步发展为肝癌。
Objective To observe interleukin-1β （IL-1β）, interleukin 6 （IL-6）, tumor necrosis α （TNF-α） in hepatocirrhosis of rats model and to study this immune functional developing process in hepatocirrhosis. Methods 70 Wistar rats were divided into 2 groups： normal group and experimental group. Then detect the index including interleukin-1β （IL-1β）, interleukin 6 （IL-6）, tumor necrosis Factor α （TNF-α） changes in5,7,9and11weeks.Results Compared with normal group, in experimental group, serum IL-1β, IL-6 and TNF-α levels were elevated with significantly difference. Conclusion Hepatocellular carcinoma in a rat model of cirrhosis of the liver immune cell factor IL-1 beta, IL-6 and TNF - a change, promote the further development of liver cirrhosis.
出处
《新疆医科大学学报》
CAS
2011年第12期1359-1361,1365,共4页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区高校科研计划项目(编号:XJEDH-2008530)
关键词
二乙基亚硝胺
肝硬化
动物模型
免疫
diethylnit ro samine
hepato cirrhosis
animal mode
immune
