摘要
目的研究长期电磁辐射对大鼠学习记忆能力的影响。方法将通过八臂迷宫训练的16只健康成年清洁级Wistar雄性大鼠随机分为辐射组和对照组,每组8只。辐射组进行功率密度为10 mW/cm2的电磁辐射,每天6 h,每周6 d,连续8周。每周采用八臂迷宫实验测定大鼠的学习记忆能力。8周后,测定大鼠脑组织乙酰胆碱酯酶活力。结果辐射组大鼠每次迷宫实验耗时标准化数值、参考记忆错误次数以及工作记忆错误次数在第1～6周明显多于对照组大鼠(P<0.05),但第7、8周辐射组大鼠与对照组大鼠在迷宫实验耗时标准化数值、参考记忆错误次数以及工作记忆错误次数上并无明显差异。辐射组和对照组大鼠脑组织乙酰胆碱酯酶活力间比较,差异无统计学意义。结论 10 mW/cm2电磁辐射在短期可使大鼠的学习记忆能力下降,但长期作用对大鼠的学习记忆能力的影响可能有限。
Objective To study the effects of long-term electromagnetic radiations on learning and memory function of rats. Methods After successfully trained in the 8-Arm maze,all rats were randomly divided into control group and radiation group. Then the radiation group was administered with 10 mW/cm^2 microwave exposure for eight consecutive weeks （six hours every day）. Every week their learning and memory abilities were tested by 8-Arm maze. After eight weeks,chemical colorimetric assay was used to detect the acetylcholinesterase activity of all the rats according to kit instructions. Results From the 1st week to the 6th week,the normalized time,reference memory error and working memory error of the radiation group in the maze test increased compared to those of control group （P〈0.05）, but from the 7th week to the 8th week they had no difference from the control group. After eight weeks,the brain acetylcholinesterase activity of control group and radiation group were （1.08± 0.13） and （1.01±0.15）U/mg Pro respectively. There had no differences between the two groups. Conclusion After 10 mW/cm^2 electromagnetic radiation, the capability of learning, spatial reference memory and working memory could be attenuated in the short term. But the effect of long-term electromagnetic radiation may be limited.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第12期1059-1062,共4页
Journal of Environment and Health
基金
国家重点基础研究发展计划("973计划"
2011CB503700)
国家自然科学基金(81071231)
北京市教委项目(JC015999201001
JX015999201001)
中南民族大学学术团队经费(XTZ09010)
关键词
电磁辐射
学习
记忆
八臂迷宫
乙酰胆碱酯酶
Electromagnetic radiation
Learning
Memory
8-Arm Maze
Acetylchoiinesterase
