摘要
目的探讨过氧化物酶体增殖物激活受体-1(peroxisomeproliferator—activatedreceptor-γ,PPAR-γ)在大鼠慢性胰腺炎(CP)进程中的表达及其意义。方法经大鼠尾静脉-次性注射二丁基二氯基锡方法制备CP模型。按体重随机分为对照组及造模后1、3、5、7、14、42d组。行胰腺常规病理检查,SiriusRed染色观察胶原含量,测定胰腺组织髓过氧化物酶(MPO)活性,免疫组化法测定仪-SMA、PPAR-γ蛋白的表达。结果造模7d内胰腺呈急性炎症改变,42d时出现不同程度的腺泡坏死、萎缩,淋巴细胞、单核细胞浸润,小叶内或小叶周围纤维化形成,伴胰管改变,符合从急性胰腺炎(AP)向CP的进展过程。造模后1d,胰腺组织MPO活性、α—SMA蛋白表达即显著增加[(0.78±0.71)U/g比(0.15±0.05)U/g,6.67±3.14比0,P值均〈0.05],之后随造模时间延长未继续增加。造模后7d,胶原含量达峰值,为(45.424-15.99)%,较对照组的(10.87±2.28)%显著增加(P〈0.05),胶原沉积从仅在血管壁进展到沉积在导管周围至小叶内和(或)小叶周围。对照组PPAR-γ仅在血管壁呈阳性表达,表达量为0.17±0.41,随造模时间延长表达渐增强,42d达峰值,为4.83±2.71。结论在CP造模过程中PPAR-γ蛋白表达逐渐增强,并发挥有限的抗炎症和抗纤维化作用。
Objective To observe the expressions of PPAR-γ,protein during the course of pancreatic fibrosis of chronic pancreatitis(CP)in Wistar rats and its significance.Methods Bibutyhin dichloride(DBTC,8 mg/kg body weight)in a volume of 200 ml solvent was injected into the tail vein to establish the CP rat's model.Wistar rats were randomly divided into control group and 1,3,5,7,14,42 d group according to weights.Pancreatic tissue underwent routine pathological examination,and collagen accumulation in pancreatic sections was determined by staining for Sirius Red.Pancreatic myeloperoxidase(MPO)activity was determined.Expressions ofα-SMA and PPAR-γ,proteins were assessed by immnnohistoehemical method.Results Light microscopy showed signs of acute pancreatitis with interstitial edema and infiltration of neutrophilic granulocytes 7 d after DBTC injection.Acinar ceils necrosis,atrophy,lymphocytes and monocytes infiltration,fibrosis within lobule and peri-lobule as well as pancreatic duct changes were found,which was in accord with the course from AP to CP.One days after induction,the activity of MPO,expressions ofα-SMA was significantly increased[(0.78±0.71)vs(0.15±0.05)U/g,6.67±3.14 vs 0 P〈0.05],then it did not increase with time of induction.Seven days after induction,collagen level reached the peak[(45.42±15.99)%],which was significantly higher than teat in control group((10.87±2.28)%,P〈0.05].Collagen fibers accumulated from periduetal to intra-acinar and/or inter-acinar areas.In control rats,the expression of PPAR-γprotein was positive only in vessel walls,and the expression level was 0.17±0.41 and increased with time of induction,then reached the peak of 4.83±2.71 at 42 d.Conclusions During the course of pancreatic fibrosis in rats,the expression of PPAR-γprotein is gradually increased,and plays a limited anti-inflammation and anti-fibrosis role.
出处
《中华胰腺病杂志》
CAS
2011年第6期426-429,共4页
Chinese Journal of Pancreatology
