摘要
评价快速血浆反应素环状卡片试验(RPR)、梅毒酶联免疫吸附试验(TP-ELISA)和梅毒螺旋体明胶凝集试验(TPPA)在无偿献血者梅毒筛查中的应用价值,选择适用于血站血液筛查梅毒的检测方法。选择无偿献血者20200份血液,采用RPR和TP-ELISA方法检测梅毒抗体,经初筛、复检阳性标本再以TPPA法确认。结果表明:RPR法阳性率为0.45%(91/20200),TP-ELISA法阳性率为0.767%(155/20200),阳性检出率差异有统计学意义(χ2=16.55,P<0.01)。经TPPA法复检,阳性标本分别为61例和137例,与RPR符合率为67.0%(61/91,P<0.01);与TP-ELISA符合率为88.4%(137/155,P>0.05)。综合考虑方法学和实际操作的可行性,TP-ELISA阳性者需经TPPA法确认。TP-ELISA法是目前最适合血站无偿献血者梅毒筛查首选方法。
To explore the application values of RPR,TP-ELISA and TPPA in syphilis screening in voluntary blood donors,and to choose a suitable model for screening syphilis in blood center.RPR and TP-ELISA were used to screen samples of 20200 blood donors for treponema pallidum（TP） infection and these positive samples were tested by TPPA.The results showed that the positive rates of RPR and TP-ELISA were 0.45% and 0.77%,respectively.There was significant difference between RPR and TP-ELISA（P〈0.01）.There were 61 and 137 positive samples screened by TPPA,and the coincidences between TPPA with RPR and TP-ELISA were 67.0% and 88.4%,respectively.The study indicates that TP-ELISA is the optimal method in detecting syphilis for massive screening of blood donors.
出处
《标记免疫分析与临床》
CAS
2011年第6期372-374,共3页
Labeled Immunoassays and Clinical Medicine
