摘要
用溴化乙锭(EB)作为荧光探针研究了各种茶叶多糖、多酚类化合物对DNA的保护作用.测定了龙井、普耳茶、乌龙茶等8种茶叶中多糖和多酚类提取物存在下DNA与EB混合液的荧光积分强度,并把不同茶叶与DNA相互作用的常数定义为结合常数D,根据D的大小讨论了多糖及多酚类化合物的存在DNA保护作用的影响.结果表明,8种茶叶与DNA有较好的作用,但作用程度不同.D值越大,茶叶对DNA的保护作用越好.多糖保护作用顺序如下:祁门红茶>绿茶(安徽)>普尔茶>乌龙茶>福建政和红茶>寿宁绿茶>信阳毛尖>龙井;多酚保护作用顺序如下:祁门红茶>福建政和红茶>寿宁绿茶>龙井>乌龙茶>绿茶(安徽)>信阳毛尖>普尔茶.
The protection action of tea polysaccharide and polyphenols on DNA are investigated using EB as a fluorescent probe in the work.The fluoresence integral intensity of DNA and EB mixture are determined in the presence of polysaccharide and polyphenols in extracts of 8 Chinese teas,such as Longjing tea,Puer tea and Oolong tea etc.D is defined as the interaction constant of different teas with DNA.According to the size of D,the impaction of protection action of tea polysaccharide and polyphenols on DNA was discussed.The result shown that the 8 kinds of teas can interact with DNA,but the degree of interaction is different.The bigger the D was,the stronger the intreraction of teas and DNA was.The order of protection action of tae polysaccharide was Qimen black tea Green tea（Anhui）Puer teaOolong teaBlack tea of Fujian ZhengheShouning green teaXinyang needle tea Longjing tea.The order of protection action of tea polyphenols was Qimen black teaBlack tea of Fujian ZhengheShouning green tea Longjing teaOolong teaGreen tea（Anhui） Xinyang needle teaPuer tea.
出处
《分子科学学报》
CAS
CSCD
北大核心
2011年第6期407-411,共5页
Journal of Molecular Science
基金
山西省国际科技合作资助项目(2010081020)
山西省归国留学人员科研基金资助项目(2008-90)
关键词
荧光探针
DNA
茶叶多糖
茶叶多酚
fluorescent probe
DNA
tea polysaccharide
tea polyphenol
