摘要
由5-溴-2′-脱氧尿苷通过1,2,4-三唑和硫代乙酸在温和的条件下合成4-硫-5-溴-2′-脱氧尿苷,再通过单溴二胺(monobromobimanes,mBBR)对5-溴-4-硫-2′-脱氧尿苷4位硫的亲电反应,合成了用单溴二胺(monobromobimanes,mBBR)标记的5-溴-4-硫-2′-脱氧尿苷,并利用1HNMR核磁共振、质谱和紫外光谱对它们的结构进行了表征,并对它的荧光光谱进行分析和研究.此化合物可以产生极高的荧光特性,由此提供了一条对人体中5-4-硫-2′-脱氧尿苷的检测途径.
5-bromor-4-thio-2′-Deoxyuridine was synthesized by 1,2,4-triazole and thio-acetic acid from 5-bromor-2′-Deoxyuridine in mild condition,and then was carried out electrophilic reaction with monobromobimanes（mBBR）at 4 position sulphur,5-bromor-4-thio-2′-Deoxyuridine labeled with monobromobimanes（mBBR） was synthesized.Their structures were analyzed and characterized using 1 H NMR,MS,UV and fluorescence spectra.It can produce high fluorescence characteristics of the compound 5-bromo-4-thiodiamine-2′-deoxyuridine,which provides a body of 5-bromo-4-thio-2′-deoxyuridine-sentive detection method.
出处
《分子科学学报》
CAS
CSCD
北大核心
2011年第6期397-401,共5页
Journal of Molecular Science
基金
教育部留学回国人员科研启动基金资助项目
