摘要
目的:研究正常体重和肥胖C57BL/6小鼠血浆纤溶酶原激活物抑制物-1(PAI-1)水平、内脏和皮下脂肪组织mRNA的表达情况及罗格列酮(RSG)的干预效果。方法:雄性C57BL/6小鼠按体重随机分为对照组、高脂组和RSG组,分别喂饲不同饲料12周,测定血脂、血清PAI-1水平及附睾、皮下脂肪组织PAI-1mRNA的表达情况。结果:RSG组小鼠体重显著高于高脂组和对照组,RSG干预可降低总甘油三酯水平。高脂组血清PAI-1水平显著高于对照组,RSG干预可降低血清PAI-1水平。3组小鼠附睾脂肪组织PAI-1mRNA表达均显著高于皮下脂肪组织;RSG干预可显著降低皮下脂肪组织PAI-1表达,而对附睾脂肪组织却无明显影响。结论:正常体重与肥胖小鼠血清PAI-1水平及内脏、皮下脂肪组织PAI-1的表达均存在差异,RSG有助于改善血脂、降低血清PAI-1水平及皮下脂肪组织PAI-1 mRNA表达。
Objective： To study the level of serum plasminogen activator inhibitor 1（PAI-1） and expression of PAI-1 mRNA in visceral and subcutaneous adipose tissues of C57BL/6 mice with normal weight or obesity and the effect of rosiglitazone（RSG） treatment. Methods： Male C57BL/6 mice were divided into 3 groups randomly.Mice in control group were fed with chow diet, mice in high-fat group were fed with high fat diet and mice in RSG group were firstly fed with high fat diet for 8 weeks and then supplemented with rosiglitazone for other 4 weeks.12 weeks later,serum total triglyceride,total cholesterol and PAI-1 level were detected and the expression of PAI-1 mRNA in adipose tissues was also measured. Results： The body weight of mice in RSG group was significantly higher than that in high-fat group and control.RSG treatment could decrease serum total triglyceride.High-fat diet could increase serum PAI-1 level,but RSG treatment could decrease PAI-1 level of mice with high fat feeding.The expression of PAI-1 mRNA of epididymal adipose tissues in all groups was all significantly higher than subcutaneous adipose tissues.RSG treatment could decrease the expression of PAI-1 mRNA in subcutaneous adipose tissues,but had no effect in epididymal adipose tissues. Conclusion： The level of serum PAI-1 and the expression of PAI-1 mRNA were different between visceral and subcutaneous adipose tissues in both normal and obese mice.RSG treatment contributed to the decrease of blood lipid,serum PAI-1 level and the expression of PAI-1 mRNA in subcutaneous adipose tissues.
出处
《现代医学》
2011年第6期653-657,共5页
Modern Medical Journal
关键词
血浆纤溶酶原激活物抑制物-1
内脏脂肪
皮下脂肪
罗格列酮
高脂饮食
小鼠
plasminogen activator inhibitor 1
visceral adipose tissue
subcutaneous adipose tissue
rosiglitazone
high-fat diet
mice
