摘要
使用降钙素基因相关肽(CGRP)干预压力超负荷心肌肥厚大鼠,探讨CGRP与心肌组织中一氧化氮、内皮素含量变化的关系以及CGRP对心肌肥厚的影响,期望进一步揭示心肌肥厚的可能机制。采用腹主动脉缩窄的方法复制压力超负荷心肌肥厚大鼠模型。选取雄性Wistar大鼠40只,随机分为4组:对照组,缩窄组,CGRP组,卡托普利组(每组N=10)。术后一周开始CGRP组给予CGRP 8μg/(kg.d-1),卡托普利组给予卡托普利30 mg/(kg.d-1)。给药4周后处死动物,检测各项指标,光镜下观察心室肌组织结构变化,分别用放射免疫方法和硝酸还原法测定心室肌ET、NO的水平。与对照组相比的结果是缩窄组大鼠SBP、LVW/BW及心肌中ET水平明显升高(P<0.05),NO明显降低(P<0.05);干预组与缩窄组相比,SBP、LVW/BW及心肌中ET水平明显降低(P<0.05),心肌中NO水平升高(P<0.05),ET水平与左心系数呈正相关。光镜下观察结果:缩窄组与对照组比较,可见心肌纤维横径明显增粗,细胞核肥大,深染,异型,心肌纤维间隙增宽,间质纤维结缔组织增生明显。CGRP组和卡托普利组心肌纤维横径及细胞核较缩窄组缩小,间质增生程度较缩窄组明显减轻形态已经与对照组相近。说明:(1)心肌中NO水平下降、ET水平上调可能参与了压力超负荷性心肌肥厚的发生发展。(2)CGRP具有一定的逆转高血压心肌肥厚的作用,此作用与临床常用抗高血压药物卡托普利相近。
To investigate the relationship between calcitonin gene-related peptide（CGRP） and nitric oxide and endothelin（ET） in myocardial tissue,and the impact of CGRP on cardiac hypertrophy of rats.Then the possible mechanism of cardiac hypertrophy is revealed.Hypertrophic myocardium was reproduced in rats by constricting abdominal aorta.40 male Wistar rats were randomly divided into four groups： control group（N=10）,coarctation group（N=10）,CGRP group（N=10）,captopril groups（N=10）.One week after surgery,CGRP group received CGRP 8 μg/（kg·d-1） and captopril group was given captopril 30 mg/（kg·d-1）.The rats were killed 4 weeks later.The indicators were detected.Changes in ventricular structure were observed in the light microscope and ET,NO levels of ventricular muscle were measured respectively with radioimmunoassay and nitric acid reduction method.It is compared with control group,SBP,LVW / BW and myocardial ET levels of rats in constriction group were significantly higher（P0.05） and NO significantly decreased（P0.05）.Compared with the constriction group,SBP,LVW / BW and myocardial ET levels were significantly lower in the intervention group（P0.05）and level of NO in the myocardium（P0.05） were higher.There was a positive correlation between myocardial NO-a,ET and left ventricular mess index.The observations in the light microscope： compared with the control group,in the constriction group,myocardial fiber diameter was significantly thicker,nuclear hypertrophy,deep staining,abnormal and myocardial fiber gap widened.And the fibrous connective tissue of stroma increased.In CGRP group and captopril group,myocardial fiber diameter and nucleus were narrower than the constriction group.The degree of proliferation of quality significantly decreaed in the constriction group.Patterns have been similar to the control group.It is conclused that：（1） Reduction in the level of NO in the myocardium and increased level of ET may be involved in the development of pressure overload cardiac hypertrophy.（2） CGRP has a certain reversal effect of hypertensive cardiac hypertrophy and the effect is similar to captopri,the commonly used anti-hypertensive drugs in clinic.
出处
《科学技术与工程》
2011年第35期8711-8715,共5页
Science Technology and Engineering
关键词
降钙素基因相关肽
心肌肥厚
一氧化氮
内皮素
逆转
calcitonin gene related protein cardiac hypertrophy nitric oxide endothelin reversion
