摘要
目的:采用气相色谱法同时测定醒脑静注射液中龙脑、麝香酮及有关物质樟脑和异龙脑四种组分的含量。方法:采用HP-INNOWAX毛细管色谱柱(30m×0.5mm,1.0μm),FID检测器。进样口温度230℃,检测器温度280℃,采用程序升温。结果:四种组分在毛细管色谱柱上具有较好的分离效果,分离度(R)均大于4。樟脑、异龙脑、龙脑和麝香酮线性范围分别为:0.010 24～0.1024mg·ml^(-1),0.00556～0.0556mg·ml^(-1),0.09124～0.9124mg·ml^(-1),0.01436～0.1436mg·ml^(-1)(r≥0.9996),均呈现良好的线性关系;平均回收率分别为96.7%,101.84%,97.8%,96.3%,RSD均≤3%(n=6)。结论:本方法简便、快速,准确、可靠,可同时测定醒脑静注射液中龙脑、麝香酮的含量及其有关物质。
Objective： To develop a GC method to determine the contents of muscone,borneol and related substances in Xingnao- jing injection. Method： An HP-INNOWAX capillary column （30 m × 0. 5 mm, 1.0 μm） was used with FID as the detector, the injector temperature was 230 ℃ and the detector temperature was 280 ℃, and column temperature raised by programming. Result： The calibra- tion curves were linear within the range of 0. 010 24-0. 102 4 mg·ml^-1 for camphor,0. 005 56-0. 055 6 mg·ml^-1 for （ ＋ ） -isoborneol, 0. 091 24-0. 912 4 mg·ml^-1 for borneol, and 0. 014 36-0. 143 6 mg·ml^-1 for muscone （ r≥ 0. 999 6 ）. The average recovery was 96. 7% ,101.84% ,97. 8% and 96. 3% ,respectively with RSD≤3% （n =6）. Conclusion： The method is simple,rapid and accurate with good reproducibility, and it is suitable for the determination of muscone, borneol and related substances in Xingnaojing injection.
出处
《中国药师》
CAS
2011年第11期1607-1609,共3页
China Pharmacist
