摘要
目的:评价5种免疫与感染相关抗体联合检测在稽留流产患者中的诊断价值。方法:采用ELISA法检测177例稽留流产患者和156例正常妊娠妇女血清中的5种抗体水平(抗心磷脂抗体、抗精子抗体、抗子宫内膜抗体、Torch IgM抗体和Torch IgG抗体),对单一和联合检测结果进行流行病学分析后进行评价并确定最佳抗体组合。结果:每种抗体水平在稽留流产患者血清中的阳性率均显著高于对照组(P<0.05),单一抗体检测的灵敏度较低,在10.2%～48.0%之间,约登指数也较低,在0.089～0.416之间;抗体联合检测的诊断价值高于单一抗体检测,其中5种抗体组合联合检测为最佳组合,灵敏度为89.3%,特异度为85.3%,约登指数为0.746,一致率为87.4%,阳性似然比为6.07,阴性似然比为0.125,阳性预测值为87.3%,阴性预测值为87.5%。结论:联合检测5种免疫与感染相关抗体对稽留流产具有较高的诊断价值,可提高稽留流产的检出率,有助于早期诊断。
Objective：To evaluate the value of combined detection of immunity and infection-related antibodies in diagnosis of missed abortion. Methods：ELISA was used to detect the levels of five kinds of antibodies（anticardiolipin antibody,antisperm antibody,anti-endometrium antibody,Torch IgM antibody and Torch IgG antibody） in serum samples of 177 patients with missed abortion and 156 normal pregnant women,the results of single detection and combined detection were analyzed by epidemiological analysis and assessed,then the combination of optimal antibodies was confirmed. Results：The level of every antibody in serum of missed abortion group was significantly higher than that in control group（P〈0.05）.The sensitivity of single antibody detection was low（10.2%~48.0%）,Youden＇s index was also low（0.089~0.416）.The diagnostic value of combined detection of antibodies was higher than that of single antibody detection.The combined detection of five kinds of antibodies was the optimal combination,the sensitivity,specificity,Youden＇s index,coincidence rate,positive likelihood ratio,negative likelihood ratio,positive predictive value and negative predictive value were 89.3%,85.3%,0.746,87.4%,6.07,0.125,87.3% and 87.5%,respectively. Conclusion：Combined detection of 5 kinds of immunity and infection-related antibodies has a high diagnostic value,which can increase the detection rate of missed abortion,and the method is helpful to early diagnosis.
出处
《中国妇幼保健》
CAS
北大核心
2011年第35期5559-5561,共3页
Maternal and Child Health Care of China
关键词
稽留流产
诊断技术
感染
抗体
Missed abortion
Diagnostic technique
Infection
Antibody
