摘要
目的探讨妊娠期接触苯对其子鼠免疫功能的影响。方法 40只孕鼠被随机等分为空气对照组和5.0、10.01、5.0 mg/m3三个不同浓度的苯染毒组,各组从孕7 d开始,连续染毒至分娩,每天染毒2 h。分别在分娩后1 d及7d,每组取5只母鼠和5只子鼠取血检测T淋巴细胞亚群和血清免疫球蛋白的含量。结果在子鼠出生后1 d和7 d,等浓度组的母鼠和子鼠的血T淋巴细胞亚群、血免疫球蛋白的含量差异均无统计学意义(P>0.05);低浓度苯染毒组与对照组比较,CD3+、CD4+、CD4+/CD8+和血各种免疫球蛋白的含量差异均无统计学意义(P>0.05);但在中、高浓度组CD3+、CD4+和CD4+/CD8+和血各种免疫球蛋白的含量均较对照组低(P<0.05),并呈明显的剂量-效应关系(P<0.01)。但无论是母鼠还是子鼠,在两个时间点间的T淋巴细胞亚群和血各种免疫球蛋白的含量变化差异无统计学意义(P>0.05)。结论妊娠期鼠吸入苯可抑制母鼠及其子鼠细胞免疫和体液免疫功能。
Objective To explore the effect of benzene exposure during pregnancy on blood T lymphocyte subset and serum immunoglobulin in mother generation and offspring mice. Methods Forty pregnant mice were divided averagely into 4 groups at random.On the 7th day after pregnancy,each of group was exposed to benzene vapour until to parturition（0.0 mg/m3,5.0 mg/m3,10.0 mg/m3 and 15.0 mg/m3,respectively,with 2 hours per day）.On the 1st and 7th days after parturition,5 mother generation mice and 5 offspring mice were killed in each group to detect blood T lymphocyte subset and blood serum immunoglobulin by flow cytometric analysis and ELISA assay. Results On the 1st and 7th days after parturition,the blood T lymphocyte subset and blood serum immunoglobulin of mother generation mice and offspring mice in middle-and high-concentration benzene groups were inhibited significantly（P0.05） in a concentration-dependent manner（P0.01）.There was a significant correlation between immunosuppression of mother generation mice and offspring mice. Conclusions Benzene exposure during pregnancy can damage immunological function of mother generation mice and offspring mice.
出处
《实用预防医学》
CAS
2011年第12期2379-2381,共3页
Practical Preventive Medicine
基金
湖南省教育厅资助项目(No.09C141)
衡阳市科技局资助项目(No.2009ks28)
关键词
苯
小鼠
妊娠
呼吸道染毒
免疫毒性
Benzene
Offspring mice
Pregnancy
Respiratory exposure
Immunotoxicity
