摘要
组织培养耐盐筛选技术可为抗逆性基因选择提供另一种方法。本研究采用9个番茄栽培品种,以不同浓度NaCl对子叶外植体、种籽发芽和植株作盐协迫处理。研究结果,21个试验设计均反映出基因间差异。经各指标测定结果,参试品种‘罗城1号’耐盐力最强,‘方茄’最弱。二品种在子叶离体培养,NaCl浓度为10g/l,一周后便可显出差异。同一浓度处理的愈伤组织芽分化亦较易反映品种间耐盐力高低。
The expression of salt tolerance in vitro could provide additional methods for selecting stress resistant genotypes. Salt tolerance screening was performed in vitro on cotyledon explants, and on seeds and whole plants of nine tomato cultivars ( Lycopersicon esculentum Mill. ) which are widely utilized in China. Genotypic differences for salt tolerance were found in all traits analyzed. A positive correlation between in vitro and plant responses was evident, whereas genotype rank based on seed germination in presence of salt was not much correlated with ranks obtained by in vitro or plant cultures. According to explant elongation , callus fresh weight, shoot regeneration, plant growth, plant succulence, number and weight of fruits per plant, 'Lotren' seemed to be the most between 'Lotren' and 'Local'the mnst susceptible one. Differences between 'Lotren' and 'Local' for explant elongation on a medium supplemented with 10 g/1 NaCl were already visible after one week of in vitro culture. This opens the way to in vitro salt tolerance screening of either crossbred lines or germplasm accessions.
出处
《园艺学报》
CAS
CSCD
北大核心
1990年第4期299-303,共5页
Acta Horticulturae Sinica
关键词
番茄
栽培
组织培养
耐盐
筛选
Lycopersicon esculentum
Tomato
Salt stress
In vitro culture
