摘要
目的探讨均相酶增强免疫(Emit)法监测丙戊酸的方法评价及其在癫痫患者中的临床意义。方法采用均相酶增强免疫法测定质控品和癫痫患者血清丙戊酸浓度,并对测定结果进行分析。结果均相酶增强免疫法测定丙戊酸的精密度;批内变异系数(CV)值为2.52%～5.70%,批间CV值为2.70%～4.85%;回收率为98.4%～105.4%,平均102.1%。210例癫痫患者391例次丙戊酸血药浓度测定中,205例次(52.4%)丙戊酸血药浓度在有效治疗范围(50～100mg/L)内;152例次(38.9%)血药浓度小于50mg/L;34例次(8.7%)血药浓度大于100mg/L。结论均相酶增强免疫法监测丙戊酸浓度简便、可行、快速,是一种理想的检测方法;合理设定丙戊酸有效血药浓度范围(50～100mg/L),临床治疗过程加强丙戊酸血药浓度监测,是保证疗效和安全的重要措施。
Objective To explore homogeneous enzyme enhancement of the immune（Emit） methods of evaluation for valproic acid （VPA） and its significance in epilepsy sufferers. Methods The results of Emit for serum control materials and VPA concentrations in patients with epilepsy were analyzed. Results The precision of VPA was determined by Emit. The value of intra CV was 2.52%- 5.7%. CV values were between 2.7%and 4.85 %. Recovery rate was 98.4%-105.4% ,with an average of 102.1~/00. 205 cases （52.4%） of serum concentrations of VPA in 391 cases in 210 patients with epilepsy, VPA plasma concentration were in the effective therapeutic range （50 -100 μg/ mL） ; plasma concentration of 152 patients （38.9%） was 〈50 μg/mL; plasma concentration of 34 cases （8. 7%） was 〉100 μg/mL. Conclusion Emit monitoring of VPA plasma concentration is an ideal way which is convenient and fast. VPA plasma concentration range of valid settings between 50 and 100 μg/mL is much more reasonable. Be sides,enhancement of the clinical treatment of VPA plasma concentration monitoring is important to ensure efficacy and safety measures.
出处
《检验医学与临床》
CAS
2011年第24期2959-2960,2963,共3页
Laboratory Medicine and Clinic
