摘要
目的从健康人的外周静脉血中获得人类基因组DNA,扩增出抗菌肽LL-37的全长基因,插入原核表达载体pQE-30以获得重组表达载体pQE-30/LL-37。方法用NaI法从健康人外周静脉血中提取人类基因组DNA,然后以它为模板PCR扩增得到LL-37全长基因,酶切回收后连接。结果得到了较高品质的人类基因组DNA,扩增得到了LL-37全长基因,成功构建了重组表达载体pQE-30//LL-37。结论用生物方法生产抗菌肽是一种很有希望的产业,而抗菌肽LL-37的重组载体的构建成功为以后进一步的实验打下了基础。
Objective To derive human whole-genome DNA from peripheral venous blood of healthy people,to amplify the full-length genes of LL-37, to insert the prokaryotic expressing vector pQE-30 and to obtain the recombinant expression vector pQE-30/LL-37. Methods To derive human whole-genome DNA from the peripheral venous blood of healthy person by the means of NaI, and then the full-length genes of LL-37 will be amplified from the template of the acquired hu- man whole-genome DNA. After the reabstraction of the restriction endonuclease from the agarose ,LL-37 will be bonded with pQE-30. Results High quality human whole-genome DNA is obtained, and the recombinant vector pQE-30/LL-37 is constructed successfully. Conclusion Producing antimicrobial peptide LL-37 by the biologic method may be a promising industry, as the construction of antimicrobial peptide recombinant vector pQE-30/LL-37 can serve as a foundation to the further research.
出处
《中南医学科学杂志》
CAS
2011年第6期643-645,共3页
Medical Science Journal of Central South China
