摘要
目的建立高效液相色谱-电喷雾串联质谱法测定人血浆中厄洛替尼的浓度。方法 血浆样品经甲醇蛋白沉淀后,以甲醇-(0.1%甲酸+5mmol.L-1 NH4Ac)(60∶40,v/v)为流动相,采用Ultimate XB-C18(4.6mm×150mm,5μm)色谱柱进行分离,流速为0.9mL·min-1,通过电喷雾离子化串联质谱,以多反应监测(MRM)方式进行检测。结果厄洛替尼的线性范围为1～2 000ng·mL-1,平均方法回收率在103.5%～109.0%,日内和日间变异均<15%。结论本法简单、快速、灵敏、重现性好,成功应用于厄洛替尼的血药浓度监测。
Objective To develop a liquid chromatography-tandem mass spectrometry to determine erlotinib in human plasma. Methods After protein precipitation with methanol, erlotinib and internal standard were chromatographically separated on an Ultimate XB C18 column （4.6 mm×150 mm, 5μm） with the mobile phase containing （0.1% formate and 5 mmol · L^-1 ammonium acetate buffer） and acetonitrile （40 ： 60, v/v） at 0. 9 mL · min^-1. The electronic spray ion tandem mass spectrum with the positive mode and multitude reaction monitor （MRM） were used to detect the analytes. Results The linear range of the calibration curves was 1-2 000 ng · mL^-1 for erlotinib. The intraand interday precision was less than 15%. The methodology recovery ranged from 103.5% to 109.0%. Conclusion The present method is sensitive, effective and reliable, which has been successfully used in the pharmacokinetic studies of erlotinib after oral administration in healthy Chinese volunteers.
出处
《中南药学》
CAS
2011年第11期801-804,共4页
Central South Pharmacy
基金
广东省科技计划(编号:2010B031500031)
广州市科技计划(编号:2010YI-C931)
关键词
厄洛替尼
高效液相色谱质谱串联法
电喷雾
erlotinib
liquid chromatography-tandem mass spectrometry
electronic spray ion
