摘要
目的:检测Hela细胞雄激素受体(AR)基因外显子1的甲基化水平。方法:用亚硫酸氢盐克隆测序法和联合亚硫酸氢盐的限制酶法(COBRA)检测不同来源Hela细胞AR基因外显子1的甲基化水平,BiQAnalyzer软件分析测序结果。结果:在非CpG胞嘧啶转化率>98%的前提下,Hela细胞AR基因外显子1片段的总甲基化率≥96.2%,除第8个CpG位点外,其他CpG位点均为完全甲基化或高甲基化。COBRA法与测序法结果一致。结论:DNA甲基化可能是Hela细胞AR基因失活的分子机制。
Aim：To investigate the methylation level of the androgen receptor（AR）exon-1 in Hela cells.Methods：Methylation analysis of the AR exon-1 was performed by bisulfite genomic sequencing（BGS）and combined bisulfite restriction analysis（COBRA）.The sequencing data were analyzed by BiQ Analyzer.Results：At relatively high conversion rate （98%）,the methylation ratio of the AR exon-1 was still more than 96.2%.Except for the eighth CpG,all other CpGs under investigations were fully or highly methylated.The results of COBRA were in accordance with that of BGS.Conclusion：DNA methylation may involve in the inactivation of AR gene in Hela cells.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第6期856-858,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省重点科技攻关基金资助项目082102310097
河南科技大学科研基金资助项目2007QN013
