摘要
目的:观察自发性高血压大鼠(SHR)心肌的血管紧张素转化酶(ACE)和ACE2的表达,以探讨ACE2和ACE在高血压发生发展中的变化。方法:取15只SHR,处死,分离左心室,行RT-PCR、Western blot蛋白质免疫印迹和免疫组织化学检测ACE及ACE2表达;同步取10只WKY大鼠作为正常血压对照组。结果:SHR组心肌ACE的mRNA和蛋白质表达都显著高于WKY组[(1.68±0.34)∶(0.33±0.12),P<0.05;(1.21±0.14)∶(0.71±0.11),P<0.05],而ACE2的mRNA和蛋白质表达皆明显低于WKY组[(0.50±0.15)∶(1.16±0.24),P<0.05;(0.71±0.24)∶(1.22±0.14),P<0.05)]。免疫组织化学染色显示,SHR组ACE的阳性率明显高于WKY组(87%∶50%,P<0.05),而ACE2的阳性率明显低于WKY组(27%∶70%,P<0.05)。结论:SHR心肌ACE明显升高,ACE2显著降低;SHR高血压发生发展过程中存在着ACE和ACE2表达的失衡。
Objective：To investigate the cardiac expression of angiotensin-converting enzyme（ACE） and ACE2 in spontaneously hypertensive rats（SHR）,whereby to explore the changes of ACE2 and ACE in hypertension.Method：Fifteen SHR were killed and the left ventricles were dissected carefully.RT-PCR,Western blot protein staining and immunohistochemistry stainning were performed to detect the cardiac expression of ACE and ACE2.Ten WKY rats served as the normotensive control group.Result：Compared with the normotensive WKY,the cardiac expression of both mRNA and protein of ACE in SHR were obviously increased（[1.68±0.34] vs [0.33±0.12],P0.05;[1.21±0.14] vs [0.71±0.11],P0.05）,whereas the cardiac levels of both mRNA and protein of ACE2 in SHR were significantly decreaed（[0.50±0.15] vs [1.16±0.24],P0.05;[0.71±0.24] vs [1.22±0.14],P0.05）.The positive rate of immunohistochemistry for cardiac ACE was markedly higher（87% vs 50%,P0.05） while the positive rate of immunohistochemistry for cardiac ACE2 distinctly lower（27% vs 70%,P0.05） in SHR than their counterparts in WKY.Conclusion：In SHR,the expression of cardiac ACE was remarkably increased,whereas ACE2 was notably decreased.There is a imblance in the expression of ACE and ACE2 in the initiation and development of hypertension.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2011年第12期914-917,共4页
Journal of Clinical Cardiology
