摘要
目的了解肠球菌临床分离株中耐万古霉素肠球菌(VRE)的表型和基因型,了解耐万古霉素肠球菌在河南地区的流行情况,以期指导临床合理用药。方法收集2007年至2010年肠球菌临床分离株832株,用Phoenix100自动鉴定系统对分离株进行鉴定;依2011年CLSI琼脂筛选法进行初筛,用K-B法测定青霉素、氨苄西林、120μg庆大霉素、环丙沙星、左氧氟沙星、利福平、红霉素、米诺环素、奎奴普丁/达福普汀、氯霉素、四环素和利奈唑胺的药敏;用Etest法测定万古霉素和替考拉宁MIC值;通过PCR方法检测万古霉素耐药基因型。结果共检出5株耐万古霉素肠球菌,且均为屎肠球菌,其对青霉素、氨苄西林、120μg庆大霉素、环丙沙星、左氧氟沙星、利福平和红霉素均耐药;对米诺环素、奎奴普丁/达福普汀、氯霉素、四环素和利奈唑胺均敏感。5株的基因型均为vanA,其中1株为vanB基因表型。结论已发现5株耐万古霉素肠球菌,临床应该合理使用抗生素,以防止VER的暴发流行。
Objective To investigate the phenotype and genotype and epidemic situation of vancomycin-resistant Enterococcus（VRE） from clinical isolates in the Henan region in order to guide clinical rational use of antibiotics.Methods Collected 832 clinical Enterococcus species from 2007 to 2010;identified isolates by Phoenix100 automatic identificating system.Selected isolates in Brain-Heart infusion agar according to CLSI 2011.Antimicrobial susceptibility testing was carried out by means of Kirby-bauer（KB）,including Penicillin,Ampicillin,Gentamicin120μg,Ciprofloxacin,Levofloxacin,Rifampicin,Erythrocin,Minocycline,Quinupristin/Dalfopristin,Chlormycetin,Tetracycline and Linezolid;Minimal inhibitory concentrations（MIC） of vancomycin and teicoplanin were examinated by Etest method;Detected Vancomycin resistant genes by PCR method.Results Found five VRE of vanA genotype,all of which were Enterococcus faecium,and were resistant to Penicillin,Ampicillin,Gentamicin120μg,Ciprofloxacin.Levofloxacin.Rifampicin and Erythrocin,and susceptible to Minocycline,Quinupristin/Dalfopristin,Chlormycetin,Tetracycline and Linezolid.Five VRE were vanA genotype,of which one was vanB phenotype.Conclusions Found five VRE,clinicians shound use antibiotics retionally in order to prevent the outbreak of VRE.
出处
《医药论坛杂志》
2011年第20期33-35,40,共4页
Journal of Medical Forum
关键词
万古霉素耐药肠球菌
基因型
表型
Vancomycin-resistant Enterococcus
Genotype
Phenotype
