摘要
以2005年度河南省征集的地方小麦新品种为材料,将其划分为3类粒质,利用近红外光谱分析法和GB/T国标法进行品质测定,用国标法校正近红外光谱品质分析数据。研究表明,近红外小麦品质测定结果与国标法测定结果相关分析和差异显著性分析均达极显著水平,说明近红外测定结果需经进一步校正方能作为准确结果进行参考;2种方法对不同粒质品质测定结果的差异显著性不同,基本趋势是随角质程度增加,参试小麦主要品质性状近红外测定准确性下降,差异增大,因此,对不同粒质品种品质结果需依据不同的校正方程进行校正。不同粒质材料经2种方法测得的结果经Sigma plot、SPSS等软件分析和最优方程拟合,得到各自的一元二次校正方程,方程校正后,近红外品质测定蛋白质含量的校正值与国标法测定结果吻合程度较好,湿面筋含量次之,而沉降值吻合程度较差,因此沉降值无法进行近红外准确测定。
Taking local wheat varieties with different grain hardness from some provinces in China 2005 as materials,we use near-infrared spectroscopy and the national standardized methods(GB/Ts) to test the wheat's quality.The results of traditional methods were used to correct data of near-infrared determination,with a view to improving the accuracy of the wheat's quality analysis by near-infrared.The results showed that:(i) significant difference of wheat quality results between near-infrared determination and the GB methods indicated that the near-infrared determination results should be modulated for the further conference.(ii) Although the results of two methods for quality determination were different,the basic trend was that with the increase of keratin,the accuracy of the near-infrared quality determination declined and the difference enlarged.So it was necessary to regulate the results of grain quality according on the base of different calibration equations for different grain hardness of wheat.(iii)The fitted equation for the different grain hardness materials between the results of the two methods was established with the softwares as Sigma plot and Spss etc.The corrected data of near-infrared method showed that data of protein and wet gluten can be well modulated,but the result of zeleny couldn't be corrected by the fitting equation.In summary,near-infrared determination can be used to measure wheat grain protein and wet gluten,but not for the determination of zeleny sedimentation value.
出处
《西北农业学报》
CAS
CSCD
北大核心
2011年第9期25-30,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家成果转化项目(2008GB2D000188)
河南省超级麦育种项目(081100110500)