摘要
目的探讨改良Hodge试验检测KPC型碳青霉烯酶最佳底物以及这种检测方法在临床上的应用。方法收集213株多药耐药的肠杆菌科细菌,选用亚胺培南、美罗培南、厄他培南为改良Hodge试验的不同底物筛选产碳青霉烯酶表型株;采用聚合酶链反应(PCR)及耐药基因测序分析细菌的耐药基因型。结果从213株肠杆菌科细菌中检出12株改良Hodge试验阳性株,其中7株为大肠埃希菌,2株为弗氏柠檬酸杆菌,臭鼻克雷伯菌、肺炎克雷伯菌、聚团泛菌各1株;12株改良Hodge试验阳性菌体外药敏试验结果显示,对临床常用抗菌药物均耐药;PCR法扩增出5株阳性株,分别为2株大肠埃希菌,臭鼻克雷伯菌、肺炎克雷伯菌、聚团泛菌各1株;扩增产物经基因测序Blast比对均为KPC-2型碳青霉烯酶。结论改良Hodge试验的最佳底物为厄他培南,美罗培南效果较差;5株耐碳青霉烯类抗菌药物的肠杆菌携带KPC-2型碳青霉烯酶。
OBJECTIVE To investigate the choice of optimum substrate of KPC-carbapenemase by the modified Hodge test, and the clinical application of the test. METHODS Meropenem, ertapenem were different substrates of the modified Hodge test, in order to screen the carbaperiemase phenotype; the drug-reslstant genotype was analyzed by polymerase chain reaction（PCR）. RESULTS There were twelve positive strains by the modified Hodge test from 213 strains of enterobacter, seven strains were Escherichia coli, two strains were Citrobacter friundii, one strain was Klebsiella ozaenae, the one strain was Klebsiella pneurnoniae and one was Pantoea aggiomerans; they were resistant to clinical common-used antibiotics. The gene sequence indicates the product of PCR was KPC-2. CONCLUSION Ertapenem are the optimum substrates of the screening of the modified Hodge tests five carbapenem-resistant Enterobacters have KPC-2 carbapenemase.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第24期5318-5320,共3页
Chinese Journal of Nosocomiology
基金
安徽省淮北市科研课题(20100242)
