摘要
目的了解耐万古霉素溶血葡萄球菌临床分离株对β-内酰胺类、氨基糖苷类、四环素、红霉素以及糖肽类耐药相关基因存在情况。方法采用PCR技术,对耐万古霉素溶血葡萄球菌WY05株分别扩增mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(4′,4″)、erm、tetM、vanA、vanB、vanC基因,并对van基因阳性扩增产物进行测序。结果从WY05菌株中检出vanA、mecA、acc(6′)/aph(2″)、erm、tetM 5种耐药基因,vanA基因扩增产物经测序、BLASTn比对分析,与屎肠球菌vanA序列一致。结论 WY05株除了携带vanA基因外,还携带mecA、acc(6′)/aph(2″)、erm、tetM基因,表型与遗传学特征均支持该菌株具有多药耐药特征;该vanA基因序列与美国纽约州耐万古霉素金黄色葡萄球菌(VRSA)临床分离株所携带Tn1546转座子内vanA部分序列一致。
【Abstract】OBJECTIVE To understand resistance-associated genes toβ-lactamase,aminoglycosides,tetracycline,erythromycin,and glycopeptides in vancomycin resistant Staphylococcus. METHODS Six clinically relevant antibiotics resistance genes,including mecA,acc（6′）/aph（2″）,aph（3′）-Ⅲ,ant（4′,4″）,erm and tetM,vancomycin resistance gene vanA,vanB,vanC in a clinical isolate of vancomycin resistant Staphylococcus,were amplified respectively by PCR,and the positive amplified products of van genes were sequenced. RESULTS A total of 5 resistance genes including mecA,acc（6′）/aph（2″）,erm,tetM and vanA were detected from WY05 strain.The positive amplified products of vanA was consistent with the sequence of van A in Enterococcus feces. CONCLUSION WY05 isolate carries vanA,except for mecA,acc（6′）/aph（2″）,erm,tetM gene,both the phenotype and genetic characteristics demonstrate that this isolate is multidrug-resistant;the sequence of vanA gene is consistent with part of which in Tn1546 carried by VRSA isolated from New York,US.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第24期5101-5103,共3页
Chinese Journal of Nosocomiology
基金
江苏省自然科学基金(BK2006540)
苏州市卫生局科技兴卫青年专项基金(SWQ15)
