摘要
背景:骨髓基质细胞在适宜条件下可分化为神经元和星形胶质细胞,分泌可溶性分子促进神经元存活。目的:观察骨髓基质细胞移植后缺血性脑梗死大鼠脑组织神经营养因子表达情况及其对神经功能的影响。方法:改良的Longa栓线法制作大鼠大脑中动脉缺血模型,1h后再灌注,24h后治疗组尾静脉注射3×106骨髓基质细胞,盐水对照组尾静脉注射1mL生理盐水,空白对照组不进行注射。结果与结论:在梗死后第7,14天治疗组的神经损伤评分明显低于对照组(P<0.05);治疗组神经生长因子和脑源性神经营养因子表达在各时间点均高于对照组(P<0.05)。结果显示静脉移植骨髓基质细胞可改善缺血性脑梗死大鼠神经功能,移植骨髓基质细胞后缺血脑组织中神经生长因子及脑源性神经营养因子表达促进了神经功能的恢复。
BACKGROUND：Bone marrow stromal cells（BMSCs） can differentiate into neurons and astrocytes under the appropriate conditions,and secrete soluble molecules to promote neuronal survival.OBJECTIVE：To observe the effect of test the effect of BMSCs on neurotrophic factors and neurologic function of rats with ischemic cerebral infarction.METHODS：Rats were subjected to transient middle cerebral artery occlusion and intravenously injected with 3×106 BMSCs（treatment group） and 1 mL normal saline（saline group） at 24 hours after stroke.No administration was done in blank control group.RESULTS AND CONCLUSION：Significant recovery of neurological function was found in the treatment group at 7 and 14 days after stroke in comparison with the other two groups（P 0.05）.Nerve growth factor and brain derived neurotrophic factor had a higher expression in the treatment group than the other two groups at different time points（P 0.05）.Intravenous injection of BMSCs can improve the neurologic function of rats with ischemic cerebral infarction and the increase in the expression of nerve growth factor and brain derived neurotrophic factor in the ischemic tissue plays an important role in neurological recovery.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第45期8455-8458,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
