摘要
在pH值为7.40的Tris-HCl缓冲溶液中,采用吸收光谱法、荧光光谱法以及粘度法研究了芹菜素(Ap)与鲱鱼精DNA(fsDNA)的相互作用。研究表明,Ap与fsDNA相互作用生成了结合比nAp∶nDNA=2∶1的复合物,温度300K和310K的结合常数Kb分别为1.068×104 L.mol-1和1.137×104 L.mol-1;300K温度下Ap与DNA相互作用的△rHm为1.899×103 J.mol-1,△rSm为83.475J.mol-1.K-1,△rGm为-2.306×104 J.mol-1,表明两者的结合过程为熵驱动反应。粘度测定结果进一步确定实验条件下Ap与fsDNA的作用方式为插入模式。
The interaction of apigenin with herring sperm DNA was studied with acridine orange as a fluorescence probe.The fluorescence spectra indicated that a kind of compound of apigenin and herring sperm DNA was formed at pH=7.40.The binary compound ratio was napigenin∶nDNA=2∶1;the binding constants of apigenin with herring sperm DNA compound were 1.068×104 L·mol-1（300 K） and 1.137×104 L·mol-1（310 K）,respectively.The thermodynamic parameters of the interaction were calculated as follows：△rHm=1.899×103 J·mol-1,△rSm=83.475 J·mol-1·K-1,△rGm=-2.306×104 J·mol-1 at the 300 K.The interaction of apigenin with DNA was also studied through method of viscosity.The results confirmed that the intercalation model was the major mode of the interaction between apigenin and herring sperm DNA,and the binding of apigenin with herring sperm DNA was an entropy-driven reaction.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第6期747-750,共4页
Journal of Analytical Science
基金
国家自然科学基金(No.20975081)
陕西省教育厅科研计划(No.2010JK899)
西北大学研究生交叉学科资助(07YJC09)
咸阳师范学院专项科研基金项目(10XSYK101
10XSYK205)
