摘要
目的优化推理选育条件,获得头孢菌素C高产菌株。方法根据头孢菌素C的生物合成途径和代谢调控机制设计菌种推理选育方案,原始出发菌株经紫外诱变后,采用托盘琼脂柱筛选法依次对铜离子、丙二酸及头孢菌素C耐受菌株进行选育。结果最终获得一株头孢菌素C高产菌株TBC-68071,其产量较原始菌株提高了154.6%,且高产特性稳定。结论对铜离子、丙二酸及头孢菌素C耐受的顶头孢霉菌株可以高产头孢菌素C,推理选育克服了随机筛选的盲目性,提高了菌种筛选的效率。
Objective To Optimize conditions of rational selection and obtain cephalosporin C high-producing strains. Methods An efficient rational selection method were designed according to the biosynthetic pathway of cephalosporin C and mechanism of metabolic regulation. After UV mutagenesis, cephalosporin C high-producing strains were screened by an agar column high-throughput method with the pressures of copper ions, malonic acid and cephalosporin C tolerance. Results The yield of cephalosporin C of the final selected strain TBC-68071 was increased 154.6% than the original strain, and the high-producinggenetic characteristics was stable. Conclusion The results showed that rational screening ofAcremonium chrysogenum overcame the blindness of random screening and improved the screening more efficiently.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2011年第12期889-894,951,共7页
Chinese Journal of Antibiotics
基金
国家科技支撑计划"抗生素战略品种的菌种选育研究"(No.2007BAI26B01)
山东省自然科学基金(No.Y2008D18)
关键词
头孢菌素C
推理选育
顶头孢霉
铜离子
丙二酸
Cephalosporin C
Rational screening
Acremonium chrysogenum
Copper ion and malonic acid
