摘要
目的探讨改良EVO150-8方法在批量生物检材DNA检验中的应用价值,建立一种自动化、简单、快速的DNA提取方法。方法采用改良EVO150-8自动化核酸提取纯化仪器与DNA IQ磁珠法纯化试剂盒,对各现场提取的880份血迹、烟蒂、口香糖、精斑(混合斑)、组织、骨骼、脱落细胞等常见生物检材进行DNA提取与纯化,采用Identifiler试剂盒进行扩增检验,用3130XL电泳,GeneMapper ID V3.2分析软件进行分析比对。结果在880份生物检材中,有836份检材成功获得STR分型;检验92份检材仅需时128min。结论改良EVO150-8适合批量生物检材的自动化提取。
Objective To establish a DNA extraction method with the characteristics of automatic,simple and fast by means of improved EVO150-8 method and evaluate its application value.Methods Genomic DNA of 880 biological samples including blood,cigarette,chewing,gum fine spot（mixed spot）,organization,bone and exfoliated cells was isolated and purified by using improved EVO150-8 automation nucleic acid extraction and purification equipment,and then was genotyped with the AmpFlSTR Identifiler PCR Amplification Kit and 3130xl Genetic Analyzer.Results In all of 880 biological samples,836 samples were genotyped successfully,and only 128 minutes were took for testing 92 samples.Conclusion The improved EVO150-8 method can be used in DNA automation extraction of batch samples.
出处
《中国法医学杂志》
CSCD
北大核心
2011年第6期468-469,共2页
Chinese Journal of Forensic Medicine
关键词
法医物证学
改良EVO150-8
自动化检案
forensic biological evidence
improved EVO150-8 method
automation platform
