摘要
目的 探讨P选择素在肺癌转移机制中的作用。方法 采用免疫组化SP法对48例原发性肺癌组织及18例癌旁组织P选择素的表达进行检测。结果 腺癌的P选择素阳性表达率明显高于鳞癌和癌旁肺组织(P<0.01)。在腺癌中:①P选择素的阳性表达率随TNM分期的递增而逐渐增高,Ⅲ～Ⅳ期的阳性表达率明显高于Ⅰ～Ⅱ期(P<0.05);②P选择素的表达与肺癌细胞分化程度、淋巴结转移及原发肿瘤大小无明显关系(P>0.05)。
Objective To investigate the role of P selectin in metastasis of lung cancer.Methods Expression of P selectin were examined in 48 primary lung cancer specimens by the microware enhanced immunohistochemical staining method (SP) using a rat monoclonal antibody against human P selectin, with 18 cases of adjacent non cancerous tissue as control. Results The positive rate of P selectin expression in adenocarcinoma was significantly higher than those of squamous cell carcinoma and adjacent non cancerous tissues (P<0.01). In adenocarcinoma, the expression of P selectin in stage Ⅲ and Ⅳ was significantly higher than that of stage Ⅰ and Ⅱ(P<0.01).But there were no significant correlation between the expression of P selectin and differentiation grade of tumor, lymph node metastasis, size of the primary tumor.Conclusion P selectin might be associated with vascular metastasis in adenocarcinoma.
关键词
P-选择素
肿瘤转移
肺肿瘤
P selectin Tumor metastasis Lung cancer Immunohistochemistry
