摘要
目的:从基因水平探讨抑癌基因p53 蛋白与鼻咽癌的关系。方法:本文采用Western blot 结合非放射性免疫沉淀技术,对鼻咽癌细胞株、鼻咽癌活检组织和鼻咽粘膜慢性炎症组织进行了p53 蛋白表达的研究。结果:7 株鼻咽癌细胞株均发现p53 蛋白表达异常条带。104 例鼻咽癌活检组织中有12 例出现p53 蛋白表达异常, 检出率为11.54 % 。8 例鼻咽慢性炎症活检组织中p53 蛋白表达未见异常。12 例p53 蛋白表达异常的鼻咽癌患者有2 例复发。结论:鼻咽癌组织中存在p53 蛋白表达异常,鼻咽癌的发生、发展可能与抑癌基因p53 蛋白的异常表达有关。
Objective: To study the relationship between p53 protein expression and progression of nasopharyngeal carcinoma. Methods: Human nasopharyngeal carcinoma cell line, primary nasopharyngeal carcinoma and chronic nasopharyngeal inflammation biopsy were examined for p53 protein expression by Western blot and immunoprecipitation techniques. Results:Abnormal expression of p53 protein was seen in 7 nasopharyngeal carcinoma cell lines (CNE1 , CNE2-Z, HNE1, HNE2, HNE3, SUNE1 and HK1). In the 104 nasopharyngeal carcinoma biopsies, 12 (11.54%) showed abnormal expression of p53 protein. Abnormal expression of p53 was not found in 8 biopsies of the nasopharyngeal chronic inflammation. Two recurrences were found in 12 patients with p53 protein abnormal expression. Conclusion: p53 protein expression may play a role in the occurrence and development of nasopharyngeal carcinoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第11期829-831,共3页
Chinese Journal of Clinical Oncology
基金
美国中华医学基金会(CMB) 赞助
关键词
鼻咽肿瘤
P53蛋白
基因表达
病理
Nasopharyngeal neoplasm p53 protein expression Western blot
