摘要
目的：探讨抗糖基化药物氨基胍（ＡＧ） 对高级糖基化终产物（ＡＧＥｓ） 促进大鼠胰星状细胞（ＰＳＣ）增殖和基质合成的干预作用。方法：用非同位素的溴脱氧尿苷（ＢｒｄＵ） 掺入法和时间分辨免疫荧光法测定ＰＳＣ 的增殖过程，以时间分辨免疫荧光法和免疫细胞化学法观察了ＡＧＥｓ 对大鼠ＰＳＣ 纤连蛋白合成的作用，并观察了氨基胍对上述效应的干预作用。结果：ＡＧＥｓ 能以浓度相关的方式明显增强ＰＳＣ的增殖和纤连蛋白的合成， ＡＧ 处理的ＡＧＥｓ上述效应明显减弱。结论：ＡＧ 对ＡＧＥｓ 促进ＰＳＣ增殖和基质合成有明显的抑制作用。
AIM: To investigate effects of aminoguanidine on advanced glycosylation end products(AGEs) in proliferation and matrix synthesis of cultured rat pancriatic stellate cells(PSC). METHODS: Proliferation of PSC was estimated by bromodeoxyuridine(BrdU) with time_resolved fluoroimmunoassay (TR_FIA). The fibronectin synthesis of rat PSC were observed by immunocyto chemistry and by TR_FIA. RESULTS: It was found that AGEs_BSA significantly stimulated proliferation of PSC in a dose_related manner. The total fibronectin synthesis of PSC was also increased by AGEs_BSA in a concentration_related manner. AGEs_BSA glycosylated in the presence of aminoguanidine under anaerobic conditions showed reduced or no significant effects on cellular proliferation and fibronectin synthesis. CONCLUSION: Our result is that AG can inhibit the proliferation and matrix synthesis of PSC stimulated by AGEs.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
1999年第6期339-342,共4页
Chinese Journal of New Drugs and Clinical Remedies
关键词
氨基胍
AGES
糖尿病
细胞外基质
纤连蛋白类
aminoguanidine
advanced glycosylation end products
diabetes mellitus
cultured cells
fibronectins
