摘要
提纯的水稻条纹病毒（云南宜良分离物）在电镜下的形态为多型性，但主要是宽8－10urn，长80－25O的分枝丝状体，有些为直径3urn或8urn的开环环状体，有些为13urn宽，130－190urn长的丝状体，但其基本结构应是直径3urn、长度不等的丝状体。经聚丙烯酸胺凝胶电泳分析，VRNA4编码的病害特异蛋白（SP）分子量为19．9kDa，而VCRNA3编码的外壳蛋白（CP）约为336kDa。在非变性条件下，RSV的4条ssRNAs大小分别为3‘OXIO‘（ssRNAI）、互．2XIc‘（ssR．NAZ）、0．9X10‘（ssRNA3）和0．8X10‘Da（SSRNA4），有时出现一条大小为0．58X10‘Da的单链RNA（ssRNA）；而4条dsRNAs的分子量分别为4．9X10‘（dsRNAI）、2．8X10‘（dsRNAZ）、20XIOo（dsRNA3）和1．7X10‘D。（dSRNA4）。利用制备电泳分离提纯的外壳蛋白免疫家兔，得到了高特异性的抗血清。A蛋白夹心ELISA检测结果表服RSV－CP与水稻草状矮化病毒（RGSV）CP抗血清有微弱的反应，但与RSV、RGSV的SP抗血清没有反应，而RSV－CP抗血清与RSV．SP及RGSV的SP、CP都无血清学关系，这个结果表明RGSV与RSV之间在进化上具有一定的亲缘关系。
An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some filaments of 13 nm in width and 130-190 nm in length.The basic morphism of RSV particles should be filaments of 3 nm in width and various length.By SDS-PAGE analysis, the molecular weight of disease-specific protein (SP) encoded by vRNA4 was 19. 9 kDa and tha of coat protein (CP) encoded by vcRNA3 was 33. 6 kDa. Whennucleic acid extrcted from the purified RSV was electrophoresed under nondenaturing condition, the she of four dsRNAs(designated as dsRNA1-4 in order of decreasing size) was 4. 9×106, 2. 7×106, 2.0×106 and 1. 7×106 Da, respectively, and that of four ssRNAs (designated as ssRNA1-4 in order of decreasing size) was 3. 0×106, 1. 2×106, 0. 9 ×106 and 0. 8×106 De, respectively. A fifth Segment with a size of 0. 58×106 Da identified as ssRNA5 associated with the purified virus somtimes. The antiserum against the coat protein further purifiedby preparative electrophoresis was raised and used to investigate the serological relationships between RSV-CP and RSV-SP, CP and SP of rice grassy stunt virus (RGSV) which is also amember of Tenuivirus. The results showed that RSV-CP had no serological reaction with SPof RSV and PGSV, but could weakly reat with antiserum of RGSV-CP, which confirmedthat there is distantly evolutionary relationship between RGSV and RSV.
出处
《中国病毒学》
CSCD
1999年第4期343-352,共10页
Virologica Sinica
基金
国家自然科学基金
福建省自然科学基金
