摘要
将构建的表达PKC_α反义RNA的质粒,转入人乳腺癌细胞Bcap-37中,通过筛选与检测获得PKC_α表达受到抑制的细胞。随后分析了细胞生长的血清依赖性、软琼脂成集落和致瘤性的变化,以及cyclin E和CDK2基因表达所受到的影响。结果显示PKC_α表达受到抑制后,乳腺癌细胞生长的血清依赖性增加,软琼脂成集落能力下降,细胞致瘤性被强烈抑制,说明PKC_α表达下降或活性降低伴随着乳腺肿瘤细胞恶性程度的减弱。同时,结果还表明PKC_α表达受到抑制后,导致细胞周期调控系统中的cyclin E及CDK2表达水平下降,说明PKC_α参与的信号转导系统与cyclin/CDK参与的细胞周期调控系统在功能上有着较为密切的联系。
Human breast cancer cell line Bcap-37 was stably transfected with the plasmid expressing antisense PKCa RNA, and cells, in which PKCα was inhibited due to antisense PKCa RNA, were isolated. Changes in serum-dependent growth in cell culture, cell clonogenicity in soft agar and growth in nude mice were tested, and the expressions of cyclin E and CDK2 were analyzed. After PKCa was inhibited,the cells showed that serum-dependent growth and anchorage-dependent
growth enhanced, tumorigenicity in nude mice decreased. The results suggest that less aggressive breast cancer phenotypes may be induced by inhibition of PKCa. Levels of cyclin E and CDK2 mR-NA in cells with antisense PKCa RNA were lower than those in control cell. These indicate that signal transduction system with PKCa is closely related to cell cycle control system with cyclin/ CDK in the functions.
出处
《实验生物学报》
CSCD
1999年第4期367-371,共5页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金重点课题(批准号39430080)
国家教委留学归国人员科研基金
