摘要
同尾酶是一类识别不同核苷酸序列但能酶切产生相同粘性末端的限制性内切酶，依靠同尾酶的这种特性，可以根据需要将不同的ＤＮＡ 片段进行灵活组合，获得各种排列顺序的多价表位重组疫苗．将这种方法用于疟疾多价重组ＤＮＡ 疫苗的研制；ＢＡＬＢ／ｃ 小鼠免疫实验对所得重组疫苗ＰＵ２８６的免疫原性进行了测定．
With the isocaudamers which have different recognition sequences and produce compatible cohesive ends,chimeric multi epitope Plasmodium falciparum DNA vaccines including the multiplication of the single copy epitope and the tandem linkage of different kinds of epitopes were flexibly constructed.A specific B cell response was detected by ELISA after the immunization of BALB/c mice with the chimeric antigen and demonstrated the usefulness of this strategy of constructing multi epitope DNA vaccines.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第6期974-977,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
"863"项目资助
关键词
疟疾
多价重组疫苗
同尾酶
DNA疫苗
Malaria,Multi epitope recombinant DNA vaccine,Isocaudamer,Immunization
