摘要
用FTA采样卡和普通定性滤纸采集藏绵羊血样,采用NaOH法提取血液基因组DNA,利用设计的一对引物对DRB1基因第三外显子进行扩增,通过PCR产物琼脂糖凝胶检测,对普通定性滤纸与FTA采样卡的两种提取DNA的方法进行比较,结果认为采用普通定性滤纸-NaOH法提取血液基因组DNA,NaOH的最佳洗涤浓度是25 mmol/L,采用FTA-NaOH法提取血液基因组DNA,NaOH的最佳洗涤浓度是20 mmol/L,但普通定性滤纸法提取血液基因组DNA平均成本远低于FTA采样卡,普通定性滤纸法提取血液基因组DNA具有快速、便捷、经济及高效的特点。
Tibetan Sheep blood samples were collected using FTA cards and general qualitative filter paper,and treated with NaOH method genomic DNA extracted from blood.A pair of primers were designed about DRB1 exon3 for PCR.Results showed that when the ordinary qualitative filter paper blood-NaOH extracted genomic DNA,the best washing NaOH concentration is 25 mmol/L;when FTA-NaOH extracted blood genomic DNA,the best washing NaOH concentration is 20 mmol/L.General qualitative filter paper method with FTA card method extracting blood genomic DNA can achieve the same effect,but general qualitative filter paper method extracting blood genomic DNA average cost far below the FTA card method,and general qualitative filter paper method was economical,quick,convenient and highly efficient.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第11期221-224,共4页
Biotechnology Bulletin
基金
国家高技术研究发展计划("863"计划)项目(2006AA10Z196)
国家科技支撑计划项目(2007BAD52B05)
甘肃省农业生物技术专项(GNSW-2009-24)
甘肃省重点实验室建设计划项目(085RTSA004)
