摘要
在构建大黄鱼肌肉组织cDNA文库的基础上,克隆了肝细胞肿瘤相关抗原-127基因。克隆到的基因全长1 439bp,其中5'-UTR 124 bp,3'-UTR 640 bp,编码序列675 bp,编码224个氨基酸。生物信息学分析显示,大黄鱼HCA127基因存在多个功能位点,即N端糖基化位点、蛋白激酶C磷酸化位点、酪蛋白激酶Ⅱ磷酸化位点和亮氨酸拉链结构位点。大黄鱼HCA127氨基酸序列具有较高的保守性,与斑马鱼、胡瓜鱼、大西洋鲑等多种鱼类的相似性在80%以上。在检测的9种组织中,肝细胞肿瘤相关抗原-127基因在肝、肾、肠、鳃、眼、脑组织中表达,其中在脑组织中表达最强,肝和鳃组织中次之,肾和眼组织中表达较微弱。
On the basis of constructing the full length cDNA library from the skeletal muscle of Larimichthys crocea,hepatocellular carcinoma-associated antigen gene-127 was cloned and sequenced.The acquired gene was 1 439 bp in length,including a 124 bp 5′-UTR,a 640 bp 3′-UTR,and a 675 bp coding sequence which encodes 224 amino acids.The bioinformatics analysis showed that HCA127 of Larimichthys crocea had several functional sites,including N-glycosylation site,protein kinase C phosphorylation site,casein kinase II phosphorylation site and a leucine zipper pattern.HCA127 amino acid sequence was highly conserved,and the similarities among Salmo salar,Danio rerio,Osmerus mordax and Larimichthys crocea were over 80%.In the examined nine tissues,HCA127 gene was expressed in liver,kidney,intestine,gill,eye and brain,with the highest expression in brain,the relatively lower expression in liver and gill,and the weakest in kidney and eye.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第11期125-129,139,共6页
Biotechnology Bulletin
基金
国家自然科学基金(30871916)
宁波大学大学生科技创新计划项目
关键词
大黄鱼
肝细胞肿瘤相关抗原-127基因
分子特征
组织表达
Larimichthys crocea Hepatocellular carcinoma-associated antigen gene-127 Molecular characterization Tissue expression
