摘要
以怀山药的茎尖、茎段为外植体,研究不同激素配比对山药茎尖分化及茎段快繁的影响,筛选适合于怀山药生长的培养基。结果表明:MS+BA 1mg/L+KT 2mg/L+NAA 0.2mg/L是怀山药茎尖分化较理想的培养基,成苗率较高(30%);MS+BA 1mg/L+NAA 0.5mg/L是怀山药茎段快繁最佳培养基,不定芽诱导率较高,成苗率最高,达到109.5%。添加0.01%聚乙烯吡咯烷酮(PVP)后茎段褐化现象得到了很大的改善。
The effects of different hormone combinations on yam shoot tip differentiation and propagation were studied using shoot tip and stem cut as explants,to select the medium suited for Huai yam culture.The result showed that MS＋BA 1 mg/L＋KT 2 mg/L＋NAA 0.2 mg/L was an ideal medium for Huai yam tip differentiation with its high rate of seedling production（30%）.MS＋BA 1 mg/L＋NAA 0.5 mg/L was the best medium for rapid propagation by Huai yam stem cuts with its relatively high induction rate of adventitious buds and highest rate of seedling production（109.5%）.Addition of 0.01% PVP inhibited browning remarkably.
出处
《河南农业科学》
CSCD
北大核心
2011年第11期123-125,共3页
Journal of Henan Agricultural Sciences
基金
国家公益性行业科研专项(200903022)
关键词
怀山药
茎尖
茎段
培养基
分化
脱毒
快繁
Huai yam
Stem tip
Stem cut
Medium
Differentiation
Detoxification
Rapid propagation
