摘要
目的：探讨抗不同组织酸性同功铁蛋白（ＡＩＦ）单克隆抗体（ＭｃＡｂ）的免疫学特性。方法：应用聚焦层析技术分别从人胎盘和原发性肝癌组织中分离出低ｐＩ值的ＡＩＦ，常规制备ＭｃＡｂ和进行免疫组织化学测定。结果：共筛选到５株抗人胎盘ＡＩＦ和２株抗人原发性肝癌（ＰＨＣ）ＡＩＦ的ＭｃＡｂ，即２ｆ８、４ｇ７、４ｇ８、５ｃ３、７ａ９和４ｃ９、４ｅ２。抗ＡＩＦＭｃＡｂｓ均与各自ＡＩＦ分子上不同抗原决定簇结合，其中针对不同组织ＡＩＦ的ＭｃＡb５ｃ３和４ｅ２作用于同一抗原决定簇。使用ＭｃＡｂ２ｆ８和４ｃ９，对正常肝组织、慢性肝炎与肝硬化和ＰＨＣ肝组织进行了免疫组织化学检查，其阳性率分别为２ｆ８：０、１０．０％、７６．３％；４ｃ９：０．６．７％、８１．６％。结论：人胎盘和ＰＨＣ组织ＡＩＦ中存在着共同的抗原决定簇，抗ＰＨＣＡＩＦＭｃＡｂ在免疫诊断中优于抗人胎盘ＡＩＦＭｃＡｂ。
objective: Study on immunological characteristics of McAbs against humnan acidic isoferritin (AIF) from different tissuesMethods: Preparation of AIF with low PI value by chromatofocusing technique and McAb using hybridoma technique. Results: Seven hybridomaCell lines secreting specific monoclonal antibody(McAb),that is,five against human Placental AIF(2f8,4g7,4g8,5c3,7a9)and two against Primary hepatic carcinoma (PHC ) AIF(4c9, 4e2 ), were raised. Seven Moth recognized different antigen epitopes of AIF from different tissues, butMcAb 5c3 and 4d2 recognized the same antigen epitope of AIF. LSAB immunohistochemical study using McAb 2f8 and 4c9 shown that positivestaining lates of liver tissue with normal, chronic hepatitis and cirrhosis, and PHC were 0, 10.0%,76.3% and 0,6.7%,81 .6% respectively. Conclusion:There is the same antigenic determanent between PHC AIF and placental AIF, and anti-PHC AIF McAb is better than antiplacental AIF McAb in immunodiagnosis of PHC.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第12期537-538,共2页
Chinese Journal of Immunology
