摘要
用Ｄｅｘｔｒａｎ Ｔ２０００，经环氧氯丙烷交联并氧化产生部分羧基，偶联对氨基苯甲脒制得水溶性亲和载体巨配基。配基密度为７１．４μｍ ｏｌ／ｇ。截留分子量为１０ 万的超滤膜对所制亲和载体的截留率大于９９．５％ ，但尿激酶能自由通过。将比活为５００ＩＵ／Ａ２８０的尿激酶粗品经亲和超滤得到纯化的尿激酶，比活达６６ ３００ＩＵ／Ａ２８０，纯度提高１３３ 倍，回收率达８３．４％ 。
A water soluble urokinase specific macroligand was prepared by oxidizing and cross linking Dextran T2000 simultaneously with epichlorohydrin. The pendant carboxyl groups formed were then coupled with p aminobenzamidine. The ligand density of the macroligand was 71.4μmol/g dextran. A hollow fiber ultrafilter with 100 000 MWCO nominal (molecular weight cut off) membrane showed a rejection over 99.5% for the macroligand, but no retention for free urokinase by means of batchwise affinity ultrafitration the starting crude urokinase with specific activity of 500IU/ A 280 was purified with a yield of 83.4% and a purification factor of 132.
出处
《华东理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1999年第6期563-566,共4页
Journal of East China University of Science and Technology
基金
国家自然科学基金
关键词
亲和超滤
葡聚糖
尿激酶
亲和载体
水溶性
affinity ultrafiltration
dextran T2000
urokinase
p aminobenzamidine
epichlorohydrin
