摘要
目的检测Stathmin、G3BP蛋白在鼻腔、鼻窦内翻性乳头状瘤(NIP)、下鼻甲黏膜(INCM)及鼻鳞状细胞癌(NSCC)中的表达情况,及其在NIP中可能的作用及相互关系。方法采用免疫组织化学法(PV-9000二步法)检测G3BP及Stathmin蛋白在34例NIP病理标本蜡块切片中的表达情况,并同时检测20例下鼻甲黏膜以及12例鼻鳞状细胞癌组织标本蜡块切片中两蛋白的表达作为对照,运用SPSS 13.0软件进行统计学分析,观察两指标在不同组织中的表达情况,分析其在NIP中的表达有无相关性。结果按本实验所采用计数标准,Stathmin、G3BP蛋白在NIP组织中的阳性表达率分别为52.9%、73.5%,均明显高于在下鼻甲黏膜组织中的表达率0%、0%,两者差异有统计学意义(P<0.05);Stathmin蛋白及G3BP蛋白在鼻鳞状细胞癌组织中的阳性表达率分别为75%、83.3%,均高于在NIP组织中的表达,Stathmin在两组织中的表达差异具有统计学意义(P<0.05),G3BP在两组织中的表达差异无统计学意义(P>0.05);两种蛋白在NIP中的表达具有正相关性(rs>0,P<0.001)。结论 Stathmin、G3BP蛋白在NIP组织中高表达,提示其可能与NIP的复发性、侵袭性有密切联系;Stathmin、G3BP蛋白的表达具有相关性,两者的联合检测可能对肿瘤的诊断及检测具有积极意义。
Objective To detect expressions of G3BP and Stathmin in Sinonasal inverted papilloma（NIP）and explore their roles in the development of NIP as well as their interrelationships. Method The immunohistochemical（PV-9000 two-step method） method was used to analyze protein expressions of G3BP and Stathmin in paraffin embedded tissues from 34 cases of sinonasal inverted papilloma,At the same time,expressions of these two proteins was also measured in 20 cases of inferior nasal concha mucosa and 12 cases of nasal squamous cell carcinoma as controls.SPSS13.0 was applied to determine the differences and correlation in the NIP.Results Positive expression rates of Stathmin protein and G3BP protein in sinonasal inverted papilloma were 52.9% and 73.5%,which were significantly higher than those in the inferior nasal concha mucosa（0%and 0%;P0.05）.Expression rates of Stathmin protein and G3BP protein in nasal squamous cell carcinoma were 75% and 83.3%,which were higher than those in the sinonasal inverted papilloma.Significant differences were found in expressions of Stathmin（P0.05）,but not G3BP（P 0.05）.Stathmin was positively related to expression of G3BP（rs0,P0.001）.Conclusion Stathmin protein and G3BP protein were highly expressed in sinonasal inverted papilloma.The result indicates that they may be associated with the recurrence and invasiveness of sinonasal inverted papilloma.Because Stathmin was positively related to expression of G3BP,joint detection of Stathmin and G3BP may be useful in the diagnosis and prognosis of cancer.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第11期120-124,共5页
Journal of Shandong University:Health Sciences
