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斯氏艾美尔球虫肌动蛋白解聚因子在HeLa细胞中的表达 被引量：3

Expression of Eimeria stiedae actin depolymerizing factor in HeLa cells
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摘要 为真核表达斯氏艾美尔球虫(Eimeria stiedae)肌动蛋白解聚因子(ADF)基因,本研究采用RT-PCR技术扩增ADF基因,构建pVAX-1-ADF真核重组质粒,并转染HeLa细胞。采用RT-PCR方法检测ADF mRNA转录水平、western blot检测ADF蛋白表达水平。琼脂糖凝胶电泳结果表明PCR扩增出一条大小为350 bp的特异性片段,与预期值相符。Western blot检测显示,转染pVAX-1-ADF的HeLa细胞在大约13 ku处出现特异性表达条带,与预期值相符。本研究构建了E.stiedae ADF基因真核表达质粒pVAX-1-ADF,并且在体外真核细胞中表达,为进一步寻找防治E.stiedae的疫苗候选基因奠定了基础。 To express the actin depolymerizing factor （ADF） of Eimeria stiedae in HeLa cells, the ADF gene was amplification from the E. stiedae total RNA by RT-PCR and cloned into pVAX-1. The resultant recombinant plasmid was transfected into HeLa cells with FuGENE. The results indicated that the ADF gene of E. sticdae was expressed in HeLa cells detected by RT-PCR and western blot, which would be useful for evaluating immunogenicity of ADF gene as a DNA vaccine against E. stiedae.
作者 任保彦 付成福 宫鹏涛 韩乾忠 杨举 李赫 张国才 张西臣 李建华
机构地区 吉林大学畜牧兽医学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2011年第12期936-939,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家863计划(2011AA10A215) 新世纪优秀人才支持计划资助项目
关键词 斯氏艾美尔球虫 ADF基因 真核表达 Eimeria stiedae actin depolymerizing factor gene eukaryotic expression
分类号 S852.72 [农业科学—基础兽医学]
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参考文献11

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二级参考文献23

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共引文献14

同被引文献51

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引证文献3

二级引证文献10

中国预防兽医学报
2011年 第12期

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