摘要
[目的]探讨人重组甲状旁腺素1-34(rhPTH1-34)对骨质疏松的治疗作用以及与血钙、磷代谢和生长因子的关系。[方法]用摘除大鼠双侧卵巢的方式制备骨质疏松模型,实验动物分为3个组:模型对照组(OVX组,摘除大鼠双侧卵巢不作任何处理);rhPTH1-34治疗组(PTH组,摘除大鼠双侧卵巢12周后用rhPTH1-34治疗8周);假手术组(sham组,仅切除卵巢周围的脂肪组织约3 g,术后12周纳入实验)。应用第4代双能X线骨密度仪测量大鼠股骨上段骨密度值(BMD);用ELISA法测定血清硬化蛋白(sclerostin)水平及骨钙素(BGP)浓度;用自动生化仪测定血清碱性磷酸酶(ALP)。[结果]rhPTH1-34治疗组、sham组均较OVX组股骨上段骨密度增高,组间比较差异有显著性(P<0.01)。rhPTH1-34治疗组血清BGP浓度值升高及sclerostin值降低,与OVX组比较差异有显著性(P<0.01)。各组血清钙、磷含量无明显变化,与OVX组比较差异无显著性,ALP值治疗组与OVX组无明显差异。[结论]rhPTH1-34能够预防股骨上段骨密度丢失,并且血清BGP浓度值升高及sclerostin值降低,但对Ca、P、ALP含量无明显作用。
[Objective]To observe the therapeutic effect of human parathyroid hormone1-34(rhPTH1-34)on osteoporosis of ovariectomized rats and its relation to blood calcium,phosphorus and growth factors.[Methods]Seventy-four ovariectomized(OVX)rats were divided into model control group(OVX group without any treatment),rhPTH1-34 treatment group(PTH group with treatment for 8 weeks),and sham operation group.BMD of femur were measured with Hologic dual energy X-ray 4500 W bone desitometer in rats.Serum sclerostin levels and BGP concentrations were determined by ELISA.[Results]BMD of the femur in rhPTH1-34 group and sham group were higher than those in OVX control group(P〈0.01).the serum sclerostin and BGP in rhPTH1-34 group were significantly different with OVX control group(P〈0.01),but the calcium,phosphorus and ALP had no significent difference with OVX group.[Conclusion]rhPTH1-34 can effectively prevent the loss of BMD in femur,increase the serum BGP and decrease sclerostin levels without obvious effect on calcium,phosphorus and ALP contents.
作者
富勇
唐永亮
赵承斌
张军
FU Yong;TANG Yong-liang;ZHAO Cheng-bin(Orthopedic Department,the 4 Hospital of Harbin Medical University,Harbin 150001,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2011年第23期2007-2009,共3页
Orthopedic Journal of China
基金
黑龙江省科技攻关计划(项目编号:GC07C34884)
