摘要
研究合理的水解工艺以有效提高高温豆粕的免疫活性。采用二次旋转回归法优化水解条件,以水解温度、酶添加量、底物添加量、酶解时间为影响因素,以淋巴细胞增殖指数(SI)为检测指标,运用SPSS13.0软件分析,最终用Design Expert 7.0.0软件优化出最优的水解条件。最优的工艺条件为:胰蛋白酶添加量7999.97U/g,酶解温度55.14℃,水解时间4.34h,底物添加量(底物与加水量之比)4.41%,经MTT法测定,此条件下酶解物对ConA诱导的小鼠脾淋巴细胞的免疫刺激指数最高,为1.1894。
In order to improve the immunoactivity of enzymatic hydrolysates from extruded high-temperature soybean meal,the hydrolysis of the substrate by trypsin was optimized using central composite rotary design.Spleen lymphocyte proliferation（expressed as the stimulation index,SI） was investigated with respect to four variables including hydrolysis temperature,enzyme amount,substrate concentration and hydrolysis time.Using SPSS 13.0 and Design Expert 7.0.0,the experimental data were analyzed to determine the optimal hydrolysis conditions as follows： enzyme amount of 7999.97 U/g,hydrolysis temperature of 55.14 ℃,hydrolysis time of 4.34 h,and substrate concentration of 4.41%.Under the optimal hydrolysis conditions,the ConA-induced spleen lymphocyte stimulation index was determined by MTT method to be 1.1894.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第21期161-164,共4页
Food Science
基金
国家"863"计划项目(2006AA10Z322)
黑龙江省科技厅科学技术计划项目(GB08B401-01)
关键词
高温豆粕
中心组合
胰蛋白酶
免疫活性
high-temperature denatured soybean meal
central composite design
trypsin
immunoactivity
