摘要
目的观察经甲氧基聚乙二醇-琥珀酰亚胺丙酸酯(mPEG-SPA)修饰后红细胞(RBC)CD55和CD59的表达情况,评估其对修饰后红细胞保存性能的影响。方法以mPEG-SPA体外修饰健康者RBC;观察修饰前后RBC的变形性、渗透脆性、自身溶血率、CD55和CD59,判断修饰后对RBC保存性能的影响。结果 mPEG-SPA修饰后红细胞的变形性、渗透脆性和自身溶血率虽属正常范围,但RBC的变形性略有下降、渗透脆性和自身溶血率有所增加;修饰后红细胞CD55表达基本不变,CD59的表达严重下降,其表达率接近于零。结论 mPEG-SPA修饰后红细胞CD59的表达严重降低,可能影响红细胞的保存性能,甚至影响修饰后红细胞输血后的体内存活率。
Objective To observe the effect of grafted methoxy polyethylene glycol（mPEG）-succinimid propionate（mPEG-SPA） on shielding the antigens of CD55 and CD59 on red blood cell（RBC）,and to evaluate the influence of the two antigens＇ expression on the preservation of RBC,modified by mPEG-SPA.Methods RBC from healthy persons were modified by mPEG-SPA,and the deformability,osmotic fragility,autohemolysis,rate and the expression of CD55 and CD59 were detected and compared before and after modification to evaluated the influence of modification on the preservation of RBC.Results Compared with the detected results before modification,the deformability of RBC decreased,while the osmotic fragility and autohemolysis rate increased,but were still in the normal range.The positive rate of CD55 expression had little significant change after modification,but CD59 expression sharply decreased and almost disappeared.Conclusion The CD59 expression on RBC could be seriously decreased by modification with mPEG-SPA,which might exert an influence on the preservation of RBC,even on its survival in vivo.
出处
《国际检验医学杂志》
CAS
2011年第18期2077-2078,2081,共3页
International Journal of Laboratory Medicine
基金
南京市医学重点科技发展项目(ZKX07014)
