摘要
目的利用TLC和HPLC指纹图谱技术,揭示柴胡及其制剂前后皂苷类成分的变化。方法 TLC采用高效硅胶G板,展开剂为三氯甲烷-乙酸乙酯-甲醇-水(2∶4∶2∶1),显色剂为1%对二甲氨基苯甲醛乙醇溶液-磷酸(3∶1),检视条件为365 nm;HPLC采用Waters XBridge RP18色谱柱(4.6 mm×100 mm,3.5μm),流动相为乙腈-水梯度洗脱,体积流量1.0 mL/min,检测波长208、252 nm。结果经TLC法对比柴胡经煎煮制成制剂前后,发现柴胡皂苷d可转化成柴胡皂苷b2,柴胡皂苷a与柴胡皂苷c基本没有变化;经HPLC指纹图谱对比研究,进一步证明了柴胡皂苷d向柴胡皂苷b2转化,且煎煮制成制剂后,指纹图谱中出现6个原药材中不存在的新色谱峰,大多数新色谱峰的最大吸收波长基本集中在250nm左右。结论对柴胡在煎煮制剂过程中新出现的皂苷类成分需进行深入系统的研究,将有助于揭示中药柴胡发挥功效的物质基础。
AIM To illustrate the quantitative changes of saikosaponins between Bupleuri Radix and its preparation by thin-layer chromatographic analysis and HPLC fingerprint technology.METHODS The trichloromethane-ethylacetate-methanol-water（2∶ 4∶ 2∶ 1） was used as the developer for the identification of Bupleuri Radix on silica gel G coating plate,while the chromogenic regent was 1% p-Dimethylaminobenzaldhyde.The monitoring wavelength was set at 365 nm.The HPLC analysis was performed on Waters XBridge RP18 column（4.6 mm×100 mm,3.5 μm） with acetonitrile-water as the mobile phase at a flow rate of 1.0 mL/min and detection wavelength of 208 and 252 nm.RESULTS Saikosaponins d in Bupleuri Radix was converted to saikosaponins b2 as measured by TLC and further confirmed by HPLC,while there were no differences in saikosaponins a and c between Bupleuri Radix and its preparation.Six peaks which could not be detected in Bupleuri Radix were presented at around 250 nm in the HPLC fingerprint of the preparation.CONCLUSION Further research is needed for the new saikosaponins synthesized in the extraction to explore the clinic effect of Bupleuri Radix.
出处
《中成药》
CAS
CSCD
北大核心
2011年第11期1840-1843,共4页
Chinese Traditional Patent Medicine
