摘要
利用农杆菌介导法,将35S启动子驱动的ipt基因遗传转化杜仲,分析ipt基因对杜仲遗传转化不定芽诱导的影响。结果表明:转ipt基因及空载体,外植体不定芽的分化率分别为0.96%和0.93%,差别不明显,但转ipt基因的单位外植体平均长芽数为3.1个,明显高于对照的1.9个,进而促进了杜仲遗传转化的不定芽的诱导,其不定芽诱导率可达2.95%,明显高于空载体对照的1.68%。本文首次证明:ipt基因能够促进转化困难木本植物杜仲的不定芽诱导,为通过该基因与其他功能基因结合提高杜仲遗传转化效率奠定了技术基础。
In this research,the ipt gene driven by 35S promoter was transferred into Eucommia ulmoides Oliv.via agrobacterium-mediated transformation,and the influence of exogenous gene on shoot regeneration was further investigated in the current work.The results showed that compared with control（ipt-free） line（0.93%）,no significant difference in shoot regeneration efficiency was obtained from the ipt transgenic lines（0.96%）.However,ipt gene enhanced the shoot number per explant,which was 3.1,obviously higher than that of control（1.9）.Thus ipt gene enhanced shoot inductivity of E.ulmoides,which was 2.95%,evidently high than CK 1.68%.The present research firstly prove that ipt gene can substantially enhance shoot regeneration of E.ulmoides and this lays a foundations for promoting genetic transformation efficiency of E.ulmoides.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2011年第6期90-93,共4页
Journal of Beijing Forestry University
基金
国家转基因新品种培育重大专项(2008ZX08010-003)
贵州省重大专项(黔科合重大专项字(2007)6004-6)
关键词
异戊烯基转移酶基因
遗传转化
杜仲
ipt gene
genetic transformation
Eucommia ulmoides Oliv.
