摘要
目的研究盐酸戊乙奎醚（PHC）对创伤性休克兔复苏后肠黏膜损伤的保护作用。方法采用Lamson’s法建立创伤性休克动物模型，24只健康日本长耳大白兔，随机分为对照组（Con组）、生理盐水复苏组（Ns组）、PHC处理组（PHC组）、山莨菪碱组（ANI组）。动态观察平均动脉压（MAP）和心率（HR），在休克前、休克末和复苏后不同时间点采血测定二胺氧化酶（DAO）活性和乳酸盐（ABL）浓度。实验结束后处死动物取小肠组织，光镜下检查病理学变化。结果四组动物休克前MAP、HR、血浆DAO活性、ABL浓度差异无统计学意义；复苏后各时间点，PHC组和ANI组MAP均显著回升，在复苏后各时间点，但与休克前和Con组相比差异无统计学意义。复苏后各时间点HR为NS〈PHC〈ANI但与休克前和Con组相比差异无统计学意义。休克末和复苏后各时间点，NS、PHC、ANI组血浆DAO活性、ABL浓度显著升高，与休克前、Con组相比差别均有统计学意义；休克后四个时间点，Ns组血浆DAO活性、ABL浓度又显著高于PHC组和ANI组（P〈0．05）；而PHC组和ANI组之间各时间点血浆DAO活性、ABL浓度差异无统计学意义。病理组织学检查显示PHC组和ANI组肠黏膜损伤较NS组显著减轻。结论PHC和ANI有助于稳定创伤性休克兔的血流动力学；对创伤性休克造成的肠黏膜损伤有一定的保护作用；监测血浆ABL浓度和DAO活性可以评估肠黏膜功能及损伤情况。
Objective To investigate the protective effects of penehyclidine hydrochloride on intestinal mucosa injury in rabbits with traumatic shock. Methods The animal model of traumatic shock was established by Lamson＇s method. Twenty - four rabbits were randomly divided into control group （Con group）, traumatic shock with normal saline group （NS group）, traumatic shock treated with penehyclidine hydrochloride group（ PHC group）and anisodamine （ANI group）. At pre -shock, tele - shock, four time points after resuscitation, mean arterial pressure （MAP） and heart rate （HR） were monitored, plasma DAO activity and ABL concentration were measured by spectrophotometry. The pathological changes of tissue samples of the intestinesin were observed under microscope. Results There were no significant differences of MAP at pre - shock between 4 groups. At tele - shock, the MAP of all rabbits in NS group, PHC group and ANI group significantly decreased. At each time point after resuscitation, MAP of rabbits in PHC group and ANI group significantly rose again, but the MAP of rabbits in NS group did not recover to the level of pre - shock and were significantly lower than in Con group, PHC group and ANI group. There were no significant group differences in HR at pre - shock and tele- shock. At each time point after resuscitation, the HR of rabbits in NS group were significantly decreased. The HR of rabbits in PHC group slightly decreased, but there were no significant differences compared with pre - shock and control group. There were no significant group differences in plasma DAO activity and ABL concentration at pre - shock time point. Compared with pre - shock and control group,the plasma DAO activity and ABL concentration in NS group, PHC group and ANI group were significantly increased at tele -shock and each time point after resuscitation. The plasma DAO activity and ABL concentration were significantly higher in NS group than in PHC and ANI group at four time points after resuscitation, but there were no significant difference between PHC group and ANI group. The result of pathology showed that intestinal mucosal injury of rabbits in PHC and ANI groups was significantly reduced compared with NS group. Conclusion PHC and ANI can stabilize hemodynamics in rabbits with traumatic shock. There was a protective effect on intestinal mucosal injury induced by traumatic shock. The plasma DAO activity and ABL concentration can be uesed to assess the extent of intestinal mucosa function and injury.
出处
《中国急救医学》
CAS
CSCD
北大核心
2011年第12期1084-1087,I0012,共5页
Chinese Journal of Critical Care Medicine
基金
徐州市社会发展基金资助项目（No.XM08C084）
徐州医学院附属医院科研基金资助项目（No.2011175）
