摘要
目的探讨呼吸道合胞病毒(respiratory syncytial virus,RSV)感染后,使用广谱抗生素头孢哌酮引起的咽部优势菌群清除对其气道炎症、气道高反应,以及对肺部CD11c+细胞状态的影响。方法 3周龄雌性Balb/c随机分入空白对照组、RSV感染组和RSV感染后口服头孢哌酮组。7 d后检测口服头孢哌酮对咽部优势菌群的清除作用,并对各组小鼠进行肺泡灌洗液炎症细胞分类计数、病理切片HE染色及气道高反应性检测,流式细胞术检测肺部CD11c+细胞MHC-II及共刺激分子表达。结果 1)正常小鼠咽部优势菌群为链球菌,RSV感染未影响咽部优势菌群的改变,口服头孢哌酮可有效清除链球菌;2)RSV感染后急性期支气管肺泡灌洗液中炎症细胞明显增多[(13.20±1.72)×105 vs(6.17±0.28)×105 cells/ml,P<0.05],肺部病理损伤加重,气道高反应性轻微上升,肺部CD11c+细胞上MHC-II表达较空白对照明显上调(平均荧光强度:14 205±1 519 vs 9 707±1 140,P<0.05);头孢哌酮致咽部优势菌的清除未进一步加重气道炎症及气道高反应性,但在一定程度上抑制了CD11c+细胞的成熟,表现为单个细胞表面MHC-II表达水平较单独感染RSV组降低,与空白对照无明显差异。结论 Balb/c小鼠感染RSV同时使用广谱抗生素头孢哌酮导致咽部优势菌被清除,可能减少抗原对肺部CD11c+细胞刺激,从而一定程度上影响CD11c+细胞的成熟。
To explore the effects of pharyngeal commensal bacterium clearance by oral broad-spectrum antibiotics cefoperazone on pulmonary CD11c＋ cells status after RSV infection,3-week-old female Balb/c mouse were divided randomly into mock group,RSV infection group and RSV infection plus cefoperazone treatment group.After 7 days antibiotic treatment pharyngeal commensal bacterium change was tested.At the same time,the MHC-II and co-stimulator molecular expression of pulmonary CD11c＋ cells,airway inflammation and airway hyperresponsiveness were tested.Our results showed that,oral cefoperazone treatment cleared the streptococcus,predominant pharyngeal commensal bacterium.In the acute stage of RSV infection,the airway inflammation and airway hyperresponsiveness were appeared,and pulmonary CD11c＋ cells turned into mature status： the mean fluorescent intensity of MHC-II rose obviously.Comparing with RSV infection group,cefoperazone-caused pharyngeal commensal clearance did not aggravate the airway inflammation or airway hyper responsiveness in RSV infection plus cefoperazone treatment group,but returned CD11c＋ cells back into the immature status： the expression of MHC-II fell down.In conclusion,after RSV infection,pharyngeal commensal bacterium clearance caused by broad-spectrum antibiotics could inhibit the maturation of APC,which would influence subsequent immune responsiveness,but not in the acute stage.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2011年第12期1033-1037,1042,共6页
Immunological Journal
基金
国家自然科学基金(NSFC30972698)
重庆市第二批高等学校优秀人才支持计划(2011.1-2012.12)
