摘要
目的探讨siRNA(small interfering RNA)对膀胱癌多药耐药性的逆转作用。方法设计针对MDR1基因的siRNA,转染膀胱癌T24/ADM细胞,应用RT-PCR分析MDR1 mRNA的表达,免疫印迹检测MDR1蛋白表达,流式细胞仪检测细胞内阿霉素积累量。MTT法检测阿霉素对T24/ADM细胞的半数抑制浓度(IC50)。结果 3条siRNA均能不同程度地逆转T24/ADM细胞的多药耐药性,使MDR1基因的mRNA及蛋白表达水平下调,细胞内阿霉素积累量显著增加(P<0.05)。结论 siRNA可逆转膀胱癌的多药耐药性。
Objective To investigate the reversal effect of siRNA on bladder carcinoma multidrug-resistance.Methods The siRNA of MDR1 was designed,synthesized and transfected into bladder carcinoma cell line T24/ADM.Expression level of MDR1 mRNA was determined with PCR.MDR1 protein expression was detected by western-blotting.The intracellular accumulation of ADM was assayed with flow cytometry.The 50% inhibition concentration（IC50） of doxorubicin（ADM） on T24/ADM was determined by MTT method.Results After treated with siRNA,the expression level of MDR1 mRNA and MDR1 protein in T24/ADM decreased obviously,The intracellular accumulation of ADM increased significantly（P0.05）.Conclusion siRNA can reverse the multidrug resistance of bladder carcinoma cell line T24/ADM.
出处
《实用癌症杂志》
2011年第6期555-557,共3页
The Practical Journal of Cancer
基金
湖北省教育厅科学技术研究计划重点项目(编号:D20082404)
关键词
MDR1基因
多药耐药
膀胱癌
MDR1 gene
Multidrug resistance
Bladder carcinoma cell line T24/ADM
