摘要
目的研究白芍饮片中芍药苷的含量和二氧化硫残留量的关系。方法从市场上随机采购10批白芍饮片,采用稀乙醇超声萃取提取芍药苷,然后采用高效液相测定芍药苷含量,考察其合格率。用碘滴定法测定各批白芍中的二氧化硫残留量,研究二氧化硫残留量与芍药苷含量的关系。结果 HPLC法检测芍药苷浓度0.53~212μg.mL-1的线性关系良好（r=0.9991,n=6）,平均加样回收率为99.01%,RSD值为1.12%。按《中国药典》对芍药苷含量的要求,10批白芍饮片只有3批合格,供试芍药饮片含硫量与芍药苷含量呈负相关。结论发现二氧化硫残留量对白芍中芍药苷的影响很大,经硫熏处理的白芍饮片芍药苷含量大幅下降。
OBJECTIVE To study the relationship between paeoniflorin concentration and sulphur dioxide residues in the prepare slices of Paeonia Radix randomly selected from the market.METHODS Ten samples of prepared slices of Paeonia Radix were purchased randomly from the market.Paeoniflorin in each sample was extracted via ultrasonic extraction with dilute ethanol.The Paeoniflorin concentration was then measured using HPLC.Acceptability was also examined.Sulphur dioxide residues in each sample was determined using iodometric titration.RESULTS Paeoniflorin concentration was 0.53-212μg·mL-1（r=0.9991,n=6） with good linearity.The average recovery rate was 99.01%,RSD 1.12%.According to the guidelines for Paeonia Radix in Pharmacopoeia of People′s Republic of China,only 3 of 10 randomly selected samples were qualified.We also found there was a negative correlation between paeoniflorin concentration and sulphur dioxide residues in the selected samples.CONCLUSION Sulphur dioxide residues has a negative influence on paeoniflorin concentration in Paeonia Radix.Paeoniflorin concentration is dramatically decreased in sulfur-processed Paeonia radix slices.
出处
《海峡药学》
2011年第11期36-38,共3页
Strait Pharmaceutical Journal
关键词
白芍
高效液相
芍药苷
碘滴定法
二氧化硫残留量
Paeonia Radix
HLPC：Paeoniflorin
Iodometric titration
Sulfur contentration
