摘要
[方法]样品用乙腈提取,弗罗里硅土固相萃取小柱净化,高效液相色谱仪分离,紫外234、281 nm分别检测虫酰肼和辛硫磷,外标法定量。[结果]对大白菜和土壤中的虫酰肼、辛硫磷进行不同水平的添加回收率试验,方法的平均回收率为77.18%~107.27%,相对标准偏差为1.50%~8.83%。虫酰肼在大白菜和土壤中的最低检测质量分数为0.01 mg/kg,辛硫磷在大白菜和土壤中的最低检测质量分数为0.02 mg/kg。[结论]该方法的灵敏度、准确度、精密度符合农药残留分析的要求,适合大批量样品的检测。
[Methods] A determination method of tebufenozide and phoxim residue in cabbage was established by HPLC.The sample was extracted by acetonitrile,cleaned by Florisil-SPE column and separated by high performance liquid chromatography.The tebufenozide and phoxim were detected by DAD detector at 234 and 281 nm respectively and quantified with external standard method.[Results] The average recoveries of the method ranged from 77.18-107.27%,and the relative standard deviation was 1.50-8.83%.The lowest detectable concentration of tebufenozide and phoxim in cabbage and soil samples were 0.01,0.02 mg/kg respectively.[Conclusions] The method had high sensitivity,accuracy and precision and was suitable for the detection of large numbers of samples.
出处
《农药》
CAS
北大核心
2011年第12期912-914,共3页
Agrochemicals
基金
农业部农药检定所农药残留试验项目(2009P358)
