摘要
目的:建立饿蚂蝗中总黄酮含量的测定方法。方法:采用紫外分光光度法,以槲皮素为对照品,测定波长510nm。结果:槲皮素对照品在0.020 8～0.104 g.L-1与吸光度线性关系良好(r=0.999 7),平均加标回收率为98.09%,测定结果的相对标准偏差为1.34%(n=5)。结论:该方法简便易行、现性好、结果可靠,适用于饿蚂蝗总黄酮的定量测定。
Objective：To establish ultraviolet spectrophotometry for the determination of total flavones in Desmodium Sambuse.Method： Quercetin was used as reference material,and the samples were determined at 510 nm.Result： The linear range for quercetin was 0.020 8-0.104 0 g·L-1.（r=0.999 7）,The recovery rate was 98.09%;RSD 1.34%（n=5）.Conclusion： This method was easy to handle with accuracy and reproducibility and suitable for quantitative determination of flavonoid in Desmodium Sambuse.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期58-59,共2页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省中药现代化科技产业研究开发专项:黔科合字[2010]5029号
关键词
饿蚂蝗
总黄酮
紫外分光光度法
Desmodium sambuens
total flavones
spectrophotometry
