摘要
背景:骨髓基质干细胞体外培养增殖力强、易于向成骨细胞及软骨细胞方向分化且成骨性能稳定等特点,成为骨组织工程中合适的种子细胞。目的:总结分析采用骨髓基质干细胞作为种子细胞,分析其直接移植于骨不连部位或复合支架或转基因治疗骨不连所具有的优劣势。方法:检索1992/2011西文生物医学期刊文献数据及CNKI数据库有关骨不连研究,骨髓基质干细胞分离、培养,在骨不连方面的应用,骨组织工程细胞支架方面的文献,英文检索词为"bone marrow stromal stem cells,nonunions,repairing,tissueen gineering",中文检索词为"骨髓基质干细胞,骨修复,骨不连,组织工程"。排除重复性研究,保留23篇进一步归纳总结。结果与结论:利用骨髓基质干细胞作为种子细胞,直接植入骨不连部位,或与适当的支架材料结合,或用骨髓基质干细胞作为靶细胞,导入外源目的基因诱导成骨的基因治疗来修复骨缺损的方法,给骨缺损的治疗带来光明的前景。但同时也存在骨髓基质干细胞增殖、分化合适条件难以准确确定,经皮移植自体骨髓基质干细胞植入体内后容易流失,不能在植入部位形成有效的细胞浓度,支架材料尚不能完全符合临床要求,以及如何将骨组织工程与基因治疗的方法结合起来等问题,需要进一步的研究。
BACKGROUND： Stem cells are primitive, unspecialized cells with two main characteristics： the ability for self-renewal indefinitely and the ability to differentiate into distinct lineages of mature cells including osteoblasts and chondrocytes. Simultaneously, bone marrow stromal stem cells are suitable seed cells in bone tissue engineering. OBJECTIVE： To summarize and analyze the advantage of bone marrow stromal stem cells as seed cells via direct transplantation or its complex stent or transgene for repair of nonunion. METHODS： Biomedical Bibliographic Database in Western Journals and China National Knowledge Infrastructure were searched for articles concerning bone nonunion, culture and application of bone marrow stromal stem cells to bone nonunion as well as bone tissue engineering published from 1998 to 2011. The key words were＂bone marrow stromal stem cells,nonunions,repairing, tissue engineering＂.Repetitive articles were excluded.Finally,23 articles were further summarized.RESULTS AND CONCLUSION：This article summarized the progress in nonunion and the preponderance of bone marrow stromal stem cells as seed cells,isolation and culture of bone marrow stromal stem cells,osteogenic induction of bone marrow stromal stem cells and repair of nonunion using bone marrow stromal stem cells.The methods bring light prospects for the treatment of nonunion,such as percutaneous implantation of autologous bone marrow mesenchymal stem cells or bone marrow stromal stem cells as seed cells combined with suitable stent materials,or bone marrow stromal stem cells as target cells to introduce endogenous target gene to induce osteogenic formation.However,some problems require further investigations,including purification of bone marrow stromal stem cells,proliferation and suitable condition for differentiation of bone marrow stromal stem cells,percutaneous implantation of autologous bone marrow mesenchymal stem cells in the body is easily lost,so perhaps they cannot form an effective cell concentration at the implanting site;Scaffolds do not completely meet the clinical demand,as well as how to combine bone tissue engineering and gene therapy.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第40期7569-7572,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
